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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab2724? Please let us know so that we can cite the reference in this datasheet
ab2724 has been referenced in 2 publications.
- Jung SC et al. Rapid, bidirectional remodeling of synaptic NMDA receptor subunit composition by A-type K+ channel activity in hippocampal CA1 pyramidal neurons. Neuron 60:657-71 (2008). ICC/IF; Rat. PubMed: 19038222
- Trinidad JC et al. Comprehensive identification of phosphorylation sites in postsynaptic density preparations. Mol Cell Proteomics 5:914-22 (2006). WB; Mouse. PubMed: 16452087
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot
Lane 1: unphosphorylated rat brain homogenate - ab2724
Lane 2: phosphorylated rat brain homogenate - ab2724
Lane 3: unphosphorylated rat brain homogenate - ab2725
Lane 4: phosphorylated rat brain homogenate - ab2725
![Immunocytochemistry/ Immunofluorescence - CaMKII alpha (phospho T286) antibody [22B1] (ab2724)](/ps/datasheet/images/2/ab2724/CaMKII-alpha-Primary-antibodies-ab2724-2.jpg)
Immunocytochemistry/ Immunofluorescence - CaMKII alpha (phospho T286) antibody [22B1] (ab2724)
ICC/IF image of ab2724 stained Hep cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2724, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Flow Cytometry - Anti-CaMKII alpha (phospho T286) antibody [22B1] (ab2724)](/ps/datasheet/images/2/ab2724/CaMKII-alpha-Primary-antibodies-ab2724-3.jpg)
Flow Cytometry - Anti-CaMKII alpha (phospho T286) antibody [22B1] (ab2724)
Overlay histogram showing SH-SY5Y cells stained with ab2724 (red line). The cells were fixed with 4% paraformaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2724, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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