Anti-CaMKII alpha (phospho T286) antibody [22B1] (ab2724)

Hi,

I am interested in ELISA kit for the human CAMKII alpha (ab2724) . There is no ready to use kit and I wonder if you can help me to choose anitbodies for ELISA sandwich.

I have already use the two of your products for Western Blot.

ab22609 and ab 2724 that is my target and ab60899 for quantifieng the western blot.

I would realy appreciate if you help me to choose the antibodies for running Sandwich ELISA and the plates that need for runing my expereiments.

Best Regards

ANSWER:

Thank you for contacting us.

Sandwich ELISA procedures can be difficult to optimize and tested match pair antibodies should be used. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere with the other antibody binding. Therefore, we are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested for sandwich ELISA. At this moment we do not have tested CamKII antibody pairs that can be used in sELISA. However based on the requirement of different epitope specificity I can recommend using ab54925 or ab54926 with ab89197 or ab92332. The antibodies are specific to different part of antigens so these can be used in sELISA.

Please be advised that sELISA is not a tested application so these products are not covered under Abpromise guarantee. However I can certainly offer you Abreview discount codes as these products are eligible. The discount codes will give you next primary antibody free of charge if you test these antibodies in sELISA and submit an Abreview. You may have to use one of these antibodies as detection or capture so I can provide you 2 discount codes for 2 products.

Let me know if you are interested in obtaining this offer I will then be sending you the discount codes right away.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Dear Abcam I'm writing you concerning the CaMKII and CaMKII Phospho antibody that you sent me an email about a few weeks ago. I have been very unlucky with all the CaMKII antibodies (from other companies) I have tried so far and feel I have thrown money out the window. I have heard a lot of positive things about your antibodies but would like to know whether it is possible to get samples of CaMKII and CaMKII phosp. antibody for 1 blot so that I can try them out before buying them. I know this is a lot to ask but I have bad experience with antibodies so I hope you understand my problem.

ANSWER:

Thank you for your enquiry and your interest in our products. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.