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ab22609 has been referenced in 7 publications.
Publishing research using ab22609? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-CaMKII antibody [6G9] (ab22609) at 10 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 :
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 56 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,65 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
IHC image of ab22609 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22609, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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