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Hi, I am interested in ELISA kit for the human CAMKII alpha (ab2724) . There is no ready to use kit and I wonder if you can help me to choose anitbodies for ELISA sandwich. I have already use the two of your products for Western Blot. ab22609 and ab 2724 that is my target and ab60899 for quantifieng the western blot. I would realy appreciate if you help me to choose the antibodies for running Sandwich ELISA and the plates that need for runing my expereiments. Best Regards |
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ANSWER: |
Thank you for contacting us.
Sandwich ELISA procedures can be difficult to optimize and tested match pair antibodies should be used. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere with the other antibody binding. Therefore, we are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested for sandwich ELISA. At this moment we do not have tested CamKII antibody pairs that can be used in sELISA. However based on the requirement of different epitope specificity I can recommend using ab54925 or ab54926 with ab89197 or ab92332. The antibodies are specific to different part of antigens so these can be used in sELISA.
Please be advised that sELISA is not a tested application so these products are not covered under Abpromise guarantee. However I can certainly offer you Abreview discount codes as these products are eligible. The discount codes will give you next primary antibody free of charge if you test these antibodies in sELISA and submit an Abreview. You may have to use one of these antibodies as detection or capture so I can provide you 2 discount codes for 2 products.
Let me know if you are interested in obtaining this offer I will then be sending you the discount codes right away.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-CaMKII antibody [6G9] (ab22609) at 10 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 :
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 56 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,65 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
IHC image of ab22609 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22609, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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