CaMKII antibody [6G9] (ab22609)

Product Name 

CaMKII antibody [6G9]

Product type 

Primary antibodies

Description 

Mouse monoclonal [6G9] to CaMKII

Immunogen 

Partially purified full length native rat protein.

Reacts with 
(species key)

Hu, Ms, Rat, Cow

Specificity 

This antibody detects the CaMKII alpha subunit in both phosphorylated and non phosphorylated forms. Expected reactivity also includes the delta and gamma isoforms but not the beta isoform.

Tested applications 
(see key)

ELISA, ICC/IF, IHC-Fr, IHC-P, IP, RIA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
RIA: Use at a concentration of 3 - 5 µg/ml.
WB: Use at a concentration of 0.1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 56 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Rat brain tissue extract

Cellular localization 

Cell membrane; presynaptic membrane. Cell junction; synapse.

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Heart >> Hypertrophy >> Other
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK

Relevance 

CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMK2A assembles into heterooligomeric complexes with other CAMK2 subunits.
CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

6G9

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

May be stored at 4 °C for 2 to 4 weeks but storage below -20 °C is not recommended.

References for CaMKII antibody [6G9] (ab22609)

  This product has been used in: (two most recent references)

Abbott AL et al. Identification of a translocation deficiency in cortical granule secretion in preovulatory mouse oocytes. Biol Reprod 65:1640-7 (2001). PubMed: 11717123

Erondu NE & Kennedy MB Regional distribution of type II Ca2+/calmodulin-dependent protein kinase in rat brain. J Neurosci 5:3270-7 (1985). PubMed: 4078628
If you publish research using ab22609 please let us know so that we can cite the reference on this datasheet.

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