![Western blot - CaMKII antibody [6G9] (ab22609) image](#CaMKII-Primary-antibodies-ab22609-8.jpg)
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Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
Overview
Product name
Anti-CaMKII antibody [6G9]
See all CaMKII products (10) ...
Description
Mouse monoclonal [6G9] to CaMKII
Specificity
This antibody detects the CaMKII alpha subunit in both phosphorylated and non phosphorylated forms. Expected reactivity also includes the delta and gamma isoforms but not the beta isoform.
Tested applications
IHC-Fr, IP, IHC-P, WB, RIA, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow
Immunogen
Partially purified full length native rat protein.
Positive control
In Western Blot, this antibody gave a positive signal in Human, Rat and Mouse Whole Brain Tissue Lysates. In Flow Cytometry, this antibody gave a positive signal in PC12 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
6G9
Isotype
IgG1
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Heart >> Hypertrophy >> Other
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
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Western blot - CaMKII antibody [6G9] (ab22609)
![Western blot - CaMKII antibody [6G9] (ab22609) image](/ps/datasheet/images/22/ab22609/CaMKII-Primary-antibodies-ab22609-8.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab22609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
RIA: Use a concentration of 3 - 5 µg/ml.
ELISA: Use at an assay dependent dilution.
Target
Function
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
Cellular localization
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
Target information above from: UniProt accessionQ9UQM7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Calcium/calmodulin dependent protein kinase II alpha antibody
- Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
see all
Database links
- Entrez Gene: 282162 Cow
- Entrez Gene: 525416 Cow
- Entrez Gene: 530719 Cow
- Entrez Gene: 532713 Cow
- Entrez Gene: 815 Human
- Entrez Gene: 816 Human
- Entrez Gene: 817 Human
- Entrez Gene: 818 Human
see all
Anti-CaMKII antibody [6G9] images:
Western blot - CaMKII antibody [6G9] (ab22609)
All lanes : Anti-CaMKII antibody [6G9] (ab22609) at 10 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 56 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,65 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
References for Anti-CaMKII antibody [6G9] (ab22609)
This product has been referenced in:
- Calegari VCet al. Endurance training activates AMP-activated protein kinase, increases expression of uncoupling protein 2 and reduces insulin secretion from rat pancreatic islets. J Endocrinol 208:257-64 (2011). WB; Rat.Read more (PubMed: 21212094) »
- Giassi ACet al. Glomerular nucleus of the weakly electric fish, Gymnotus sp.: Cytoarchitecture, histochemistry, and fiber connections-Insights from neuroanatomy to evolution and behavior. J Comp Neurol 519:1658-76 (2011). IHC; Fish.Read more (PubMed: 21452231) »
See all 6 publications for this product
Publishing research using ab22609? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"