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Anti-CaMKII antibody [6G9] (ab22609)

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Overview

Product name

Anti-CaMKII antibody [6G9]
See all CaMKII products (10) ...

Description

Mouse monoclonal [6G9] to CaMKII

Specificity

This antibody detects the CaMKII alpha subunit in both phosphorylated and non phosphorylated forms. Expected reactivity also includes the delta and gamma isoforms but not the beta isoform.

Tested applications

IHC-Fr, IP, IHC-P, WB, RIA, ELISAmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Predicted to work with

Cow

Immunogen

Partially purified full length native rat protein.

Positive control

In Western Blot, this antibody gave a positive signal in Human, Rat and Mouse Whole Brain Tissue Lysates. In Flow Cytometry, this antibody gave a positive signal in PC12 cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

6G9

Isotype

IgG1

  • Western blot - CaMKII antibody [6G9] (ab22609)Western blot - CaMKII antibody [6G9] (ab22609) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab22609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.

Cellular localization

Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.

Target information above from: UniProt accessionQ9UQM7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Calcium/calmodulin dependent protein kinase II alpha antibody
  • Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
  • Calcium/calmodulin dependent protein kinase II beta antibody
  • Calcium/calmodulin dependent protein kinase II delta antibody
  • Calcium/calmodulin dependent protein kinase II gamma antibody
  • calcium/calmodulin dependent protein kinase IIA antibody
  • Calcium/calmodulin dependent protein kinase type II alpha chain antibody
  • Calcium/calmodulin dependent protein kinase type II antibody
  • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
  • CaM kinase II alpha antibody
  • CaM kinase II alpha chain antibody
  • CaM kinase II alpha subunit antibody
  • CaM kinase II antibody
  • CaM kinase II beta antibody
  • CaM kinase II delta antibody
  • CaM kinase II gamma antibody
  • CaM kinase II subunit alpha antibody
  • CAMK 2 antibody
  • CAMK 2A antibody
  • CaMK II alpha subunit antibody
  • CaMK II antibody
  • CaMK-II subunit alpha antibody
  • CAMK2 antibody
  • CAMK2A antibody
  • CAMK2B antibody
  • CAMK2D antibody
  • CAMK2G antibody
  • CAMKA antibody
  • KCC2A_HUMAN antibody
  • KIAA0968 antibody
see all

Anti-CaMKII antibody [6G9] images:

  Western blot - CaMKII antibody [6G9] (ab22609)

Western blot - CaMKII antibody [6G9] (ab22609)

All lanes : Anti-CaMKII antibody [6G9] (ab22609) at 10 µg/ml

Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)

Lysates/proteins at 20 µg per lane.

Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution

Performed under reducing conditions.

Predicted band size : 56 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,65 kDa,70 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 5 minutes

References for Anti-CaMKII antibody [6G9] (ab22609)

This product has been referenced in:

  • Calegari VCet al. Endurance training activates AMP-activated protein kinase, increases expression of uncoupling protein 2 and reduces insulin secretion from rat pancreatic islets. J Endocrinol 208:257-64 (2011). WB; Rat.Read more (PubMed: 21212094) »
  • Giassi ACet al. Glomerular nucleus of the weakly electric fish, Gymnotus sp.: Cytoarchitecture, histochemistry, and fiber connections-Insights from neuroanatomy to evolution and behavior. J Comp Neurol 519:1658-76 (2011). IHC; Fish.Read more (PubMed: 21452231) »

See all 6 publications for this product

Publishing research using ab22609? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"