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Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
Anti-CaMKII antibody [EP1829Y] (ab52476)
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Related products
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab52476? Please let us know so that we can cite the reference in this datasheet
ab52476 has been referenced in 2 publications.
- Cuddapah VA & Sontheimer H Molecular interaction and functional regulation of ClC-3 by Ca2+/calmodulin-dependent protein kinase II (CaMKII) in human malignant glioma. J Biol Chem 285:11188-96 (2010). IP; Human. PubMed: 20139089
- Riccio A et al. Essential role for TRPC5 in amygdala function and fear-related behavior. Cell 137:761-72 (2009). IHC-P; Mouse. PubMed: 19450521
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - CaMKII antibody [EP1829Y] (ab52476)](/ps/datasheet/Images/52/ab52476/ab52476_1.jpg)
Western blot - CaMKII antibody [EP1829Y] (ab52476)
Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/20000 dilution + Mouse brain lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 54 kDa
Observed band size : 45,70 kDa (why is the actual band size different from the predicted?)
![Immunohistochemistry (Paraffin-embedded sections) - CaMKII antibody [EP1829Y] (ab52476)](/ps/datasheet/Images/52/ab52476/ab52476_2.jpg)
Immunohistochemistry (Paraffin-embedded sections) - CaMKII antibody [EP1829Y] (ab52476)
Immunohistochemical staining of paraffin-embedded human brain carcinoma using ab52476 at a 1/100 dilution.
![Immunocytochemistry/ Immunofluorescence - CaMKII antibody [EP1829Y] (ab52476)](/ps/datasheet/images/52/ab52476/CaMKII-Primary-antibodies-ab52476-2.jpg)
Immunocytochemistry/ Immunofluorescence - CaMKII antibody [EP1829Y] (ab52476)
ICC/IF image of ab52476 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52476, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
![CaMKII antibody [EP1829Y] for WB in Human (52476)](/ps/datasheet/images/52/ab52476/CaMKII-Primary-antibodies-ab52476-1.jpg)
CaMKII antibody [EP1829Y] for WB in Human (52476)
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