Anti-CaMKII antibody [EP1829Y] (ab52476)

human heart tissue
sees no bands
loaded up to 50 ug protein
lysis with Laemmli buffer without protease inhibitors
Ab: 1/1000, 1/500
block w/ milk
transfer is OK
secondary is OK

suggestion: add protease inhibitors to lysis buffer

ANSWER:

Thank you for contacting us.
I am sorry to hear that the antibody is giving problems.

As we discussed over the phone,I would suggest adding protease inhibitors to the lysis buffer in order to prevent the lysed proteins to be digested and degraded by proteases.

Please let me know if this helps to improve your results. If not, please let me know also your order or PO number, so that we can discuss other options of resolving the issue.

I wish you good luck and look forward to hear back from you.

Dear Technical Team, Hope this e-mail finds you well! Kindly arrange to send us the certificate of compliance (CoC) for the below Abcam products as desired by one of our customers : Cat No. Description ab97021 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) ab97049 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) ab97023 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) ab60314 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) ab97080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed ab65623 ECL Western Blotting Substrate Kit - 50 Tests ab52476 Rabbit monoclonal [EP1829Y] to CaMKII ab11800 Goat polyclonal to DCXR ab7260 Rabbit polyclonal to GFAP - Astrocyte Marker ab104224 Mouse monoclonal [1B7] to FOX3 ab76260 Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9) ab64230 Mounting Medium ab82477 Rabbit polyclonal to Adenosine A1 Receptor ab3462 Rabbit polyclonal to alpha 1 Adrenergic Receptor

ANSWER:

I am beginning to gather the CoC's you have requested. When you have a chance, please provide the lot number from each catalog number so I can include this information on the documentation. Thanks for the additional information.

Could you please let me know whether this antibody reacts with a specific subunit of CAMKII (for example alpha) or whether it recognises all subunits?

Thank you

ANSWER:

Thank you for your enquiry.

I can confirm that ab52476 CaMKII antibody [EP1829Y] recognizes all subunits of the protein, since the peptide immunogen is highly conserved between CaMKII-alpha, -beta, -gamma, and -omega.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.