![Western blot - CaMKII antibody [EP1829Y] (ab52476) image](#ab52476_1.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - CaMKII antibody [EP1829Y] (ab52476) image](#ab52476_2.jpg)
![Immunocytochemistry/ Immunofluorescence - CaMKII antibody [EP1829Y] (ab52476) image](#CaMKII-Primary-antibodies-ab52476-2.jpg)
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Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
Overview
Product name
Anti-CaMKII antibody [EP1829Y]
See all CaMKII products (10) ...
Description
Rabbit monoclonal [EP1829Y] to CaMKII
Specificity
This antibody reacts with CaMKII
Tested applications
WB, IP, ICC, Flow Cyt, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues on human CaMKII
Positive control
IHC-P: Human brain carcinoma tissue WB: Mouse brain lysate
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1829Y
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Heart >> Hypertrophy >> Other
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/20,000Detects a band of approximately 45 , 70 kDa (predicted molecular weight: 54 kDa).
IP
IP: 1/50IP: 1/50
ICC
ICC: 1/250 - 1/500.ICC: 1/250 - 1/500.
Flow Cyt
Flow Cyt: 1/80Flow Cyt: 1/80
IHC-P: 1/100 - 1/250.(Antigen retrieval recommended.)
Target
Function
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
Cellular localization
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
Target information above from: UniProt accessionQ9UQM7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Calcium/calmodulin dependent protein kinase II alpha antibody
- Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
see all
Database links
- Entrez Gene: 815 Human
- Entrez Gene: 816 Human
- Entrez Gene: 817 Human
- Entrez Gene: 818 Human
- Entrez Gene: 108058 Mouse
- Entrez Gene: 12322 Mouse
- Entrez Gene: 12323 Mouse
- Entrez Gene: 12325 Mouse
see all
Anti-CaMKII antibody [EP1829Y] images:
Western blot - CaMKII antibody [EP1829Y] (ab52476)
![Western blot - CaMKII antibody [EP1829Y] (ab52476)](/ps/datasheet/Images/52/ab52476/ab52476_1.jpg)
Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/20000 dilution + Mouse brain lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 54 kDa
Observed band size : 45,70 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - CaMKII antibody [EP1829Y] (ab52476)
![Immunohistochemistry (Paraffin-embedded sections) - CaMKII antibody [EP1829Y] (ab52476)](/ps/datasheet/Images/52/ab52476/ab52476_2.jpg)
Immunohistochemical staining of paraffin-embedded human brain carcinoma using ab52476 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - CaMKII antibody [EP1829Y] (ab52476)
![Immunocytochemistry/ Immunofluorescence - CaMKII antibody [EP1829Y] (ab52476)](/ps/datasheet/images/52/ab52476/CaMKII-Primary-antibodies-ab52476-2.jpg)
ICC/IF image of ab52476 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52476, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
References for Anti-CaMKII antibody [EP1829Y] (ab52476)
This product has been referenced in:
- Cuddapah VA & Sontheimer H Molecular interaction and functional regulation of ClC-3 by Ca2+/calmodulin-dependent protein kinase II (CaMKII) in human malignant glioma. J Biol Chem 285:11188-96 (2010). IP; Human.Read more (PubMed: 20139089) »
- Riccio Aet al. Essential role for TRPC5 in amygdala function and fear-related behavior. Cell 137:761-72 (2009). IHC-P; Mouse.Read more (PubMed: 19450521) »
See all 2 publications for this product
Publishing research using ab52476? Please let us know so that we can cite the reference in this datasheet
![CaMKII antibody [EP1829Y] for WB in Human (52476)](/ps/datasheet/images/52/ab52476/CaMKII-Primary-antibodies-ab52476-1.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"