Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
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ab32678 has been referenced in 3 publications.
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All lanes : Anti-CaMKII (phospho T286) antibody (ab32678) at 1/1000 dilution
Lane 1 : Rat brain cortex lysate.
Lane 2 : Rat brain cortex lysate preincubated with lambda-phosphatase.
Lysates/proteins at 50 µg per lane.
Predicted band size : 50 kDa
Observed band size : 50,60 kDa (why is the actual band size different from the predicted?)
Predicted molecular weight: ~50 kDa for the alpha subunit and ~60 kDa for the beta subunit of CaMKII.
These images show the phosphospecificity of this antibody.
ab32678 (2µg/ml) staining CaMKII (phospho T286) in human Brain:cortex:frontal-lateral using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartments within the stellate cells and the myelinated fibres of white matter region .
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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