CaMKII (phospho T286) antibody (ab32678)

Product Name 

CaMKII (phospho T286) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to CaMKII (phospho T286)

Immunogen 

Synthetic phosphopeptide corresponding to amino acids surrounding phospho-286 from Rat brain CaMKII.

Reacts with 

Hu, Ms, Rat, Xl

Predicted to react 
(species key)

Chk, Cow
(Due to sequence homology)

Specificity 

This antibody is specific for the ~50 kDa alpha CaM Kinase II subunit and the ~60 kDa beta CaM Kinase II subunit phosphorylated at Thr286 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000. Predicted molecular weight: ~50 kDa for the alpha subunit and ~60 kDa for the beta subunit of CaMKII.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Rat cortex lysate.

Cellular localization 

Cell membrane; presynaptic membrane. Cell junction; synapse.

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Heart >> Hypertrophy >> Other
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK

Relevance 

CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMK2A assembles into heterooligomeric complexes with other CAMK2 subunits.
CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES. pH 7.5.

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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CaMKII (phospho T286) antibody images:

References for CaMKII (phospho T286) antibody (ab32678)

  This product has been used in:

Nutt LK et al. Metabolic control of oocyte apoptosis mediated by 14-3-3zeta-regulated dephosphorylation of caspase-2. Dev Cell 16:856-66 (2009). WB; Xenopus laevis. PubMed: 19531356

If you publish research using ab32678 please let us know so that we can cite the reference on this datasheet.

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