Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
Overview
Product name
Anti-CaMKII (phospho T286) antibody
See all CaMKII products (10) ...
Description
Rabbit polyclonal to CaMKII (phospho T286)
Specificity
This antibody is specific for the ~50 kDa alpha CaM Kinase II subunit and the ~60 kDa beta CaM Kinase II subunit phosphorylated at Thr286 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Tested applications
WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Pig, Xenopus laevis
Predicted to work with
Chicken, Cow
Immunogen
Synthetic phosphopeptide corresponding to amino acids surrounding phospho-286 from Rat brain CaMKII.
Positive control
Rat cortex lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES. pH 7.5.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Heart >> Hypertrophy >> Other
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calmodulin / CaMK
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Predicted molecular weight: 50 kDa.(Predicted molecular weight: ~50 kDa for the alpha subunit and ~60 kDa for the beta subunit of CaMKII.)
IHC-P: Use a concentration of 2 µg/ml
IHC-Fr: 1/1600
Target
Function
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
Cellular localization
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
Target information above from: UniProt accessionQ9UQM7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Calcium/calmodulin dependent protein kinase II alpha antibody
- Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
see all
Database links
- Entrez Gene: 374174 Chicken
- Entrez Gene: 374175 Chicken
- Entrez Gene: 422688 Chicken
- Entrez Gene: 423737 Chicken
- Entrez Gene: 282162 Cow
- Entrez Gene: 525416 Cow
- Entrez Gene: 530719 Cow
- Entrez Gene: 532713 Cow
see all
Anti-CaMKII (phospho T286) antibody images:
Western blot - CaMKII (phospho T286) antibody (ab32678)
All lanes : Anti-CaMKII (phospho T286) antibody (ab32678) at 1/1000 dilution
Lane 1 : Rat brain cortex lysate.
Lane 2 : Rat brain cortex lysate preincubated with lambda-phosphatase.
Lysates/proteins at 50 µg per lane.
Predicted band size : 50 kDa
Observed band size : 50,60 kDa (why is the actual band size different from the predicted?)
Predicted molecular weight: ~50 kDa for the alpha subunit and ~60 kDa for the beta subunit of CaMKII.
These images show the phosphospecificity of this antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CaMKII (phospho T286) antibody (ab32678)
ab32678 (2µg/ml) staining CaMKII (phospho T286) in human Brain:cortex:frontal-lateral using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartments within the stellate cells and the myelinated fibres of white matter region .
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-CaMKII (phospho T286) antibody (ab32678)
This product has been referenced in:
- Nutt LKet al. Metabolic control of oocyte apoptosis mediated by 14-3-3zeta-regulated dephosphorylation of caspase-2. Dev Cell 16:856-66 (2009). WB; Xenopus laevis.Read more (PubMed: 19531356) »
See 1 publication for this product
Publishing research using ab32678? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"