CaV1.3 antibody (ab54421)

Product Name 

CaV1.3 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to CaV1.3

Immunogen 

Synthetic peptide: KY-DNKVTIDDYQEEAEDKD, corresponding to internal sequence amino acids 809-825 of Rat CaV1.3
BLAST 'KY-DNKVTIDDYQEEAEDKD' with or

Reacts with 

Ms, Rat

Predicted to react 
(species key)

Hm
(Due to sequence homology)

Specificity 

ab54421 recognizes all forms of the Alpha 1D-subunit of the voltage-gated calcium channel. The epitope is specific for Alpha 1D-subunits of the voltage-gated Ca²⁺ channel and is not present in any other known protein.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/200. Predicted molecular weight: 250 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Membrane. Multi-pass membrane protein.

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Channels
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Channels >> L-type

Relevance 

Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the "high-voltage activated" (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).


Alpha 1-subunits of voltage-gated Ca²⁺ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (1 µg/ml), leupeptin (1 µg/ml), aprotinin (1 µg/ml), benzamidine (0.1 µg/ml), AEBSF (0.2 mM) and calpain inhibitors I (LLNL, 8 µg/ml) and II (ALLM, 8 µg/ml).

To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Working dilutions of antibody should be made in a physiological buffer containing a carrier protein such as 1-3% BSA.

ab54421 is supplied with a control peptide (20 µg, lyophilized from PBS). Reconstitute the peptide with 100 µl dH2O, aliquot, and freeze (-20°C). Antibody binding can be fully inhibited with the control blocking peptide. For use as a negative control, preincubate 1 µg control peptide with 1 µg antibody for 1 hr at room temperature.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 5% Sucrose, 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.700 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

Notes 

20 µg control peptide lyophilized from PBS.
Reconstitute the peptide with 100 µl dH2O, aliquot, and freeze (-20°C).

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