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Product Name
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CaV1.3 antibody
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See all CaV1.3 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CaV1.3
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Immunogen
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Synthetic peptide: KY-DNKVTIDDYQEEAEDKD, corresponding to internal sequence amino acids 809-825 of Rat CaV1.3 BLAST 'KY-DNKVTIDDYQEEAEDKD' with or 
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Reacts with
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Ms, Rat
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Predicted to react
(species key)
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Hm (Due to sequence homology)
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Specificity
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ab54421 recognizes all forms of the Alpha 1D-subunit of the voltage-gated calcium channel. The epitope is specific for Alpha 1D-subunits of the voltage-gated Ca2+ channel and is not present in any other known protein.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: 1/200. Predicted molecular weight: 250 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Membrane. Multi-pass membrane protein.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Channels Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Channels >> L-type
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Relevance
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Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the "high-voltage activated" (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).
Alpha 1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (1 µg/ml), leupeptin (1 µg/ml), aprotinin (1 µg/ml), benzamidine (0.1 µg/ml), AEBSF (0.2 mM) and calpain inhibitors I (LLNL, 8 µg/ml) and II (ALLM, 8 µg/ml).
To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Working dilutions of antibody should be made in a physiological buffer containing a carrier protein such as 1-3% BSA.
ab54421 is supplied with a control peptide (20 µg, lyophilized from PBS). Reconstitute the peptide with 100 µl dH2O, aliquot, and freeze (-20°C). Antibody binding can be fully inhibited with the control blocking peptide. For use as a negative control, preincubate 1 µg control peptide with 1 µg antibody for 1 hr at room temperature.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.05% Sodium Azide Constituents: 5% Sucrose, 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.700 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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Notes
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20 µg control peptide lyophilized from PBS. Reconstitute the peptide with 100 µl dH2O, aliquot, and freeze (-20°C).
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CaV1.3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to CaV1.3
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