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Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Channels >> L-type
Overview
Product name
Anti-CaV1.3 antibody
See all CaV1.3 products (5) ...
Description
Rabbit polyclonal to CaV1.3
Specificity
ab54421 recognizes all forms of the Alpha 1D-subunit of the voltage-gated calcium channel. The epitope is specific for Alpha 1D-subunits of the voltage-gated Ca2+ channel and is not present in any other known protein.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
Hamster, Cow, Dog, Human, Chimpanzee
Immunogen
Synthetic peptide: KY-DNKVTIDDYQEEAEDKD, corresponding to internal sequence amino acids 809-825 of Rat CaV1.3
KY-DNKVTID DYQEEAEDKD
General notes
40 µg control peptide lyophilized from PBS.
Reconstitute the peptide with 100 µl dH2O, aliquot, and freeze (-20°C).
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 5% Sucrose, 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Primary antibody notes
Alpha 1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (1 µg/ml), leupeptin (1 µg/ml), aprotinin (1 µg/ml), benzamidine (0.1 µg/ml), AEBSF (0.2 mM) and calpain inhibitors I (LLNL, 8 µg/ml) and II (ALLM, 8 µg/ml). To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Working dilutions of antibody should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. ab54421 is supplied with a control peptide (20 µg, lyophilized from PBS). Reconstitute the peptide with 100 µl dH2O, aliquot, and freeze (-20°C). Antibody binding can be fully inhibited with the control blocking peptide. For use as a negative control, preincubate 1 µg control peptide with 1 µg antibody for 1 hr at room temperature.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - CaV1.3 antibody (ab54421)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/200Predicted molecular weight: 250 kDa.
Target
Function
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).
Tissue specificity
Expressed in pancreatic islets and in brain, where it has been seen in cerebral cortex, hippocampus, basal ganglia, habenula and thalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in skeletal muscle.
Sequence similarities
Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1D subfamily.
Domain
Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ01668
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- alpha-1 polypeptide antibody
- isoform 2 antibody
- L type antibody
see all
Database links
- Entrez Gene: 484724 Dog
- Entrez Gene: 776 Human
- Entrez Gene: 12289 Mouse
- Entrez Gene: 29716 Rat
- Omim: 114206 Human
- SwissProt: Q01668 Human
- SwissProt: Q99246 Mouse
- SwissProt: P27732 Rat
see all
Anti-CaV1.3 antibody images:
Western blot - CaV1.3 antibody (ab54421)
All lanes : Anti-CaV1.3 antibody (ab54421) at 1/200 dilution
Lane 1 : Rat brain lysates, membrane incubated with ab54421.
Lane 2 : Rat brain lysates, membrane incubated with ab54421 pretreated with antigenic peptide.
developed using the ECL technique
Predicted band size : 250 kDa
References for Anti-CaV1.3 antibody (ab54421)
This product has been referenced in:
- Gezen-Ak Det al. The Effects of Vitamin D Receptor Silencing on the Expression of LVSCC-A1C and LVSCC-A1D and the Release of NGF in Cortical Neurons. PLoS One 6:e17553 (2011). WB; Rat.Read more (PubMed: 21408608) »
See 1 publication for this product
Publishing research using ab54421? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"