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Anti-Calbindin antibody [CB-955] (ab82812)

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Overview

Product name

Anti-Calbindin antibody [CB-955]
See all Calbindin products (9) ...

Description

Mouse monoclonal [CB-955] to Calbindin

Specificity

This antibody is specific to a protein of 28 kDa. Calbindin-D-28K is also known as vitamin D-dependent calcium-binding protein or cholecalcin. Calbindin D-28K has a broad tissue distribution; however it exhibits a cell type-specific expression pattern. This antibody reacts specifically with calbindin-D-28K in brain and kidney tissues from human, bovine, goat, porcine, rabbit, dog, cat, rat, mouse, sheep, and guinea pig sources. It binds weakly to chicken calbindin-D-28K.

Tested applications

ICC/IF, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Sheep, Rabbit, Goat, Guinea pig, Cow, Cat, Human, Pig

Immunogen

Purified bovine kidney calbindin-D-28K

Positive control

Kidney

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

CB-955

Isotype

IgG1

Research areas

Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins

Applications

Show applications key

Our Abpromise guarantee covers the use of ab82812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent dilution. (PubMed: 20542987)

  • ShowHide

    IHC-P

     IHC-P: 1/15 - 1/30.Perfo...Read more →

    IHC-P: 1/15 - 1/30.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

Function

Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

Sequence similarities

Belongs to the calbindin family.
Contains 5 EF-hand domains.

Domain

This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.

Target information above from: UniProt accessionP05937 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • avian-type antibody
  • CAB27 antibody
  • CALB 1 antibody
  • CALB antibody
  • CALB1 antibody
  • CALB1_HUMAN antibody
  • Calbindin 1 28kDa antibody
  • Calbindin antibody
  • Calbindin D28 antibody
  • D 28K antibody
  • D-28K antibody
  • D28K antibody
  • Vitamin D dependent calcium binding protein antibody
  • Vitamin D dependent calcium binding protein avian type antibody
  • Vitamin D-dependent calcium-binding protein antibody
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Database links

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References for Anti-Calbindin antibody [CB-955] (ab82812)

This product has been referenced in:

  • Siegel Net al. Contribution of human amniotic fluid stem cells to renal tissue formation depends on mTOR. Hum Mol Genet 19:3320-31 (2010). ICC/IF; Human.Read more (PubMed: 20542987) »

See 1 publication for this product

Publishing research using ab82812? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"