Anti-Calbindin antibody [EP3478] (ab108404)

Overview

• Product nameAnti-Calbindin antibody [EP3478]See all Calbindin primary antibodies ...
• Description
  Rabbit monoclonal [EP3478] to Calbindin
• Tested applicationsWB, IHC-P, ICC more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

• Positive controlFetal brain, fetal cerebellum, fetal hypothalamus and fetal pituitary lysates; Human brain tissue
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP3478
• IsotypeIgG
• Research Areas
  • Neuroscience
  • Neurotransmission
  • Calcium Signaling
  • Calcium Binding Proteins

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Soma marker

Applications

Target

• FunctionBuffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
• Sequence similaritiesBelongs to the calbindin family.
  Contains 5 EF-hand domains.
• DomainThis protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.

Target information above from: UniProt accession P05937 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 793 Human
  • Entrez Gene: 12307 Mouse
  • Entrez Gene: 83839 Rat
  • Omim: 114050 Human
  • SwissProt: P05937 Human
  • SwissProt: P12658 Mouse
  • SwissProt: P07171 Rat
  • Unigene: 65425 Human

  • Unigene: 277665 Mouse
  • Unigene: 3908 Rat

  see all

• Alternative names
  avian-type antibodyCAB27 antibodyCALB 1 antibody

  CALB antibodyCALB1 antibodyCALB1_HUMAN antibodyCalbindin 1 28kDa antibodyCalbindin antibodyCalbindin D28 antibodyD 28K antibodyD-28K antibodyD28K antibodyOTTHUMP00000166027 antibodyOTTHUMP00000225441 antibodyRTVL H protein antibodyVitamin D dependent calcium binding protein antibodyVitamin D dependent calcium binding protein avian type antibodyVitamin D-dependent calcium-binding protein antibody

  see all

Anti-Calbindin antibody [EP3478] images

• 

  Western blot - Calbindin antibody [EP3478] (ab108404)
  All lanes : Anti-Calbindin antibody [EP3478] (ab108404) at 1/1000 dilution
  
  Lane 1 : Fetal brain lysate
  Lane 2 : Fetal cerebellum lysate
  Lane 3 : Fetal hypothalamus lysate
  Lane 4 : Fetal pituitary lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 30 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calbindin antibody [EP3478] (ab108404)
  ab108404, at 1/100 dilution, staining Calbindin in paraffin-embedded Human brain tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Calbindin antibody [EP3478](ab108404)
  ab108404 showing positive staining in Normal kidney tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Calbindin antibody [EP3478](ab108404)
  ab108404 showing negative staining in Normal heart tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Calbindin antibody [EP3478](ab108404)
  ab108404 showing negative staining in Normal colon tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Calbindin antibody [EP3478](ab108404)
  ab108404 showing negative staining in Normal liver tissue.