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Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
Overview
Product name
Anti-Calbindin antibody - Neuronal Marker
See all Calbindin products (9) ...
Description
Rabbit polyclonal to Calbindin - Neuronal Marker
Tested applications
ICC/IF, IF, ELISA, IHC-P, IP, WB, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
28 kDa calbindin-D protein purified from rat kidney
Positive control
Rat kidney extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 30mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration. (PubMed: 21345011)
IF
IF: 1/200 - 1/1000.IF: 1/200 - 1/1000.
ELISA: Use at an assay dependent dilution.
IHC-P: Use a concentration of 1 µg/ml
IP: Use a concentration of 5 µg/ml
WB: Use a concentration of 0.1 µg/mlDetects a band of approximately 28 kDa.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 19826483)
Target
Function
Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Sequence similarities
Belongs to the calbindin family.
Contains 5 EF-hand domains.
Domain
This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.
Target information above from: UniProt accessionP05937
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- avian-type antibody
- CAB27 antibody
- CALB 1 antibody
see all
Database links
- Entrez Gene: 793 Human
- Entrez Gene: 12307 Mouse
- Entrez Gene: 83839 Rat
- Omim: 114050 Human
- SwissProt: P05937 Human
- SwissProt: P12658 Mouse
- SwissProt: P07171 Rat
- Unigene: 65425 Human
see all
Anti-Calbindin antibody - Neuronal Marker images:
Western blot - Calbindin antibody - Neuronal Marker (ab11426)
Western blot of calbindin D from rat kidney using ab11426.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Calbindin antibody - Neuronal Marker (ab11426)
ab11426 at a dilution of 1/1000, staining Calbindin in neurons (Alexa 488 secondary at 1/2000) on 30
NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates). Imgae recoloured in Photoshop.
Immunocytochemistry - Calbindin antibody - Neuronal Marker (ab11426)
Mouse Embryonic Stem Cells immunopositive for Calbindin (Abcam ab11426, 1:500). Secondary antibody: Alexa Fluor 488 goat anti-rabbit IgG (1:500). Cells underwent a 5-stage differentiation protocol to generate neurons. These cells were stained after stage 5.
Hannah Taylor, Wolfson Centre for Age Related Diseases, United Kingdom
Immunohistochemistry (PFA perfusion fixed frozen sections) - Calbindin antibody - Neuronal Marker (ab11426)
ab11426 at a dilution of 1/1000, staining Calbindin in neurons (Alexa 488 secondary at 1/2000) on 30
NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
Protocols
References for Anti-Calbindin antibody - Neuronal Marker (ab11426)
This product has been referenced in:
- Minnerup Jet al. Effects of neural progenitor cells on sensorimotor recovery and endogenous repair mechanisms after photothrombotic stroke. Stroke 42:1757-63 (2011).Read more (PubMed: 21566228) »
- El-Akabawy Get al. Purmorphamine Increases DARPP-32 Differentiation in Human Striatal Neural Stem Cells Through the Hedgehog Pathway. Stem Cells Dev 20:1873-87 (2011). ICC/IF; Human.Read more (PubMed: 21345011) »
See all 6 publications for this product
Publishing research using ab11426? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"