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Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
Overview
Product name
Anti-Calbindin antibody - Neuronal Marker
See all Calbindin products (9) ...
Description
Rabbit polyclonal to Calbindin - Neuronal Marker
Tested applications
IHC-Fr, IHC-P, IHC-FoFr, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Rabbit, Hamster, Cow, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Calbindin.
(Peptide available as ab60452.)
Positive control
This antibody gave a positive signal in the following tissues: Kidney (Mouse), Kidney (Human), Brain (Rat)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/200
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FoFr: 1/300
WB: Use a concentration of 1 µg/mlDetects a band of approximately 29 kDa (predicted molecular weight: 30 kDa).
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Sequence similarities
Belongs to the calbindin family.
Contains 5 EF-hand domains.
Domain
This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.
Target information above from: UniProt accessionP05937
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- avian-type antibody
- CAB27 antibody
- CALB 1 antibody
see all
Database links
- Entrez Gene: 539930 Cow
- Entrez Gene: 793 Human
- Entrez Gene: 12307 Mouse
- Entrez Gene: 83839 Rat
- Omim: 114050 Human
- SwissProt: P04467 Cow
- SwissProt: P05937 Human
- SwissProt: P12658 Mouse
see all
Anti-Calbindin antibody - Neuronal Marker images:
Western blot - Calbindin antibody (ab49899)
All lanes : Anti-Calbindin antibody - Neuronal Marker (ab49899) at 1 µg/ml
Lane 1 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
Lane 2 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 30 kDa
Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Calbindin antibody - Neuronal Marker (ab49899)
Immunohistochemistical detection of Calbindin using antibody ab49899 on PFA perfusion-fixed frozen rat brain sections (30um). Antigen retrieval step: None Primary Antibody diluted at 1/300 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). ab49899 was used on sections of rat brain in free floating IHC; expected staining was observed on rat brain. The submitted images show staining obtained in the cerebral cortex (A,B). The images were taken using the objective X5 (A) and X40 (B) and show the neuronal profiles (arrows) and neuronal processes.
Sophie Pezet, CNRS, Paris, France
Immunocytochemistry/ Immunofluorescence - Calbindin antibody - Neuronal Marker (ab49899)
ICC/IF image of ab49899 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49899, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calbindin antibody - Neuronal Marker (ab49899)
IHC image of Calbindin staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49899, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-Calbindin antibody - Neuronal Marker (ab49899)
ab49899 has not yet been referenced specifically in any publications.
Publishing research using ab49899? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"