Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
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ab117207 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
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Hi John, I received the antibody today. Unfortunately, there has been a mistake in the new order. The antibody I received is the same as before (ab25085), while I was expecting to receive ab49889. |
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ANSWER: |
I apologize once again for my mistake in shipping you the wrong antibody. The new antibody ab49899 will be shipped on Monday and will arrive on Tuesday. Your order number is ****** |
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Bought ab25085 without realizing that it also recognized cholecalcin. What are the other calbindin options and what is there immunogen seq? |
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ANSWER: |
I am sorry that you were having issues with ab25085 in immuno staining. I have sent you the replacement antibody ab49889, which is only listed as recognizing calbindin and also is raised against a different sequence compare to ab2085. Your new order number is *******. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab25085 staining human Calbindin D28 in Human cerebellum by immunohistochemistry formalin fixed, paraffin embedded.
ICC/IF image of ab25085 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25085, 1ug/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Anti-Calbindin antibody (ab25085) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 28 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Additional bands at : 67 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
Anti-Calbindin antibody (ab25085) at 1 µg/ml +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
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