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Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
Overview
Product name
Anti-Calbindin antibody
See all Calbindin products (9) ...
Description
Rabbit polyclonal to Calbindin
Specificity
ab25085 recognises cholecalcin (vitamin D-dependent calcium-binding protein).
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Rabbit, Horse, Chicken, Hamster, Cow
Immunogen
Synthetic peptide: MCGKEFNKAFELYDQ, corresponding to amino acids 185-199 of Human Calbindin
MCGKEFNKAF ELYDQ
Positive control
Rat brain
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml.Predicted molecular weight: 28 kDa.
IHC-P: 1/400Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
Target
Function
Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Sequence similarities
Belongs to the calbindin family.
Contains 5 EF-hand domains.
Domain
This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.
Target information above from: UniProt accessionP05937
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- avian-type antibody
- CAB27 antibody
- CALB 1 antibody
see all
Database links
- Entrez Gene: 396519 Chicken
- Entrez Gene: 539930 Cow
- Entrez Gene: 793 Human
- Entrez Gene: 12307 Mouse
- Entrez Gene: 83839 Rat
- Omim: 114050 Human
- SwissProt: P04354 Chicken
- SwissProt: P04467 Cow
see all
Anti-Calbindin antibody images:
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Calbindin antibody (ab25085)
ab25085 staining human Calbindin D28 in Human cerebellum by immunohistochemistry formalin fixed, paraffin embedded.
Immunocytochemistry/ Immunofluorescence - Calbindin antibody (ab25085)
ICC/IF image of ab25085 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25085, 1ug/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Western blot - Calbindin antibody (ab25085)
Anti-Calbindin antibody (ab25085) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 28 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Additional bands at : 67 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
Western blot
Anti-Calbindin antibody (ab25085) at 1 µg/ml + Calbindin protein (ab82631) at 0.001 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
References for Anti-Calbindin antibody (ab25085)
This product has been referenced in:
- Jacquet BVet al. Specification of a Foxj1-Dependent Lineage in the Forebrain Is Required for Embryonic-to-Postnatal Transition of Neurogenesis in the Olfactory Bulb. J Neurosci 31:9368-82 (2011).Read more (PubMed: 21697387) »
- Barone Set al. Deletion of the Cl-/HCO3- exchanger pendrin downregulates calcium-absorbing proteins in the kidney and causes calcium wasting. Nephrol Dial Transplant : (2011). WB.Read more (PubMed: 21873623) »
See all 5 publications for this product
Publishing research using ab25085? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"