|
|
|
|
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Calbindin antibody (ab25085)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence - Calbindin antibody (ab25085)
(enlarge)
|
|
|
|
|
Product Name
|
Calbindin antibody
|
| |
See all Calbindin antibodies (8)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to Calbindin
|
|
Immunogen
|
Synthetic peptide: MCGKEFNKAFELYDQ, corresponding to amino acids 185-199 of Human Calbindin
BLAST 'MCGKEFNKAFELYDQ' with  or 
|
|
Reacts with
|
Hu, Ms
|
|
Predicted to react
(species key)
|
Rat, Cow, Hm, Hrs, Rb
(Due to sequence homology)
|
|
Specificity
|
ab25085 recognises cholecalcin (vitamin D-dependent calcium-binding protein).
|
|
Tested applications
(see key)
|
ICC/IF, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
IHC-P: 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Recommend boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 28 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Rat brain
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
|
|
Relevance
|
Calbindin D28k is a member of a large family of intracellular calcium binding proteins, containing EF hand calcium binding motifs, and related to calmodulin and troponin C. The biological roles of Calbindin D28k include calcium regulation and calcium dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. Calbindin antibody is often used as a marker for cerebellum Purkinje cells.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Protein G purified
|
|
Storage buffer
|
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.200 mg/ml
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Calbindin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Calbindin antibody images, references, products related to ab25085 and other tools.
|
| |
Calbindin antibody images:
|
 Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Calbindin antibody (ab25085)
ab25085 staining human Calbindin D28 in Human cerebellum by immunohistochemistry formalin fixed, paraffin embedded.
|
| |
Immunocytochemistry/ Immunofluorescence - Calbindin antibody (ab25085)
ICC/IF image of ab25085 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25085, 1ug/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
|
| |
References for Calbindin antibody (ab25085)
|
|
This product has been used in:
Mehedint MG et al. Choline deficiency alters global histone methylation and epigenetic marking at the Re1 site of the calbindin 1 gene. FASEB J 24:184-95 (2010). WB; Mouse. PubMed: 19752176
If you publish research using ab25085 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to Calbindin
|
| |
Calbindin antibody - more information
|
Customer reviews (feedback) regarding Calbindin antibody |
Customer FAQs regarding Calbindin antibody |
Protocols for Calbindin antibody |
Price and availability of products related to Calbindin antibody |
| |
Calbindin antibody - related products:
|
|
|
| |
Calbindin antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Calbindin antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
