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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calbindin antibody (ab89690)
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Product Name
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Calbindin antibody
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See all Calbindin antibodies (8)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to Calbindin
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Immunogen
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Purified Calbindin from chicken gut
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Reacts with
(species key)
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Hu, Ms, Rat, Chk, Fsh, Gpig, Hm, Mk, Rb, Shp
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/50 - 1/100 ABC method.
Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
WB: 1/500. Predicted molecular weight: 30 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse brain tissue.
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Cellular localization
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Cytoplasmic
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Research areas
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Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
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Relevance
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Calbindin D28k is a member of a large family of intracellular calcium binding proteins, containing EF hand calcium binding motifs, and related to calmodulin and troponin C. The biological roles of Calbindin D28k include calcium regulation and calcium dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. Calbindin antibody is often used as a marker for cerebellum Purkinje cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Calbindin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Calbindin antibody images, references, products related to ab89690 and other tools.
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Calbindin antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calbindin antibody (ab89690)
ab89690 at 1/50 dilution staining Calbindin in mouse brain by Immunohistochemistry, Paraffin-embedded tissue.
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Search PubMed (MEDLINE) for references to Calbindin
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Calbindin antibody - more information
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