Anti-Calcitonin antibody [16B5 ] (ab11493)

Overview

• Product nameAnti-Calcitonin antibody [16B5 ]See all Calcitonin primary antibodies ...
• Description
  Mouse monoclonal [16B5 ] to Calcitonin
• SpecificityThis antibody recognises human procalcitonin and calcitonin. Specific for calcitonin (central part).
• Tested applicationsSandwich ELISA, Flow Cyt, ELISA, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant fragment (Human).

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: PBS, pH 7.4
• Concentration information loading...
• PurityProtein A purified
• Purification notesPurity tested by electrophoresis.
• Clonality Monoclonal
• Clone number16B5
• MyelomaSp2/0
• IsotypeIgG2b
• Research Areas
  • Cancer
  • Tumor biomarkers
  • Hormones

  • Cancer
  • Growth factors
  • Hormones

  • Signal Transduction
  • Growth Factors/Hormones
  • Hormones

Applications

Target

• RelevanceCalcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.
• Cellular localizationCytoplasmic and Secreted
• Database links
  • Entrez Gene: 796 Human
  • Omim: 114130 Human
  • SwissProt: P01258 Human
  • Unigene: 37058 Human

• Alternative names
  Alpha CGRP antibodyalpha type CGRP antibodyCALC 1 antibody

  CALC A antibodyCALC1 antibodyCALCA antibodycalcitonin 1 antibodycalcitonin gene related peptide 1 antibodycalcitonin gene related peptide I antibodyCalcitonin related polypeptide alpha antibodyCGRP 1 antibodyCGRP antibodyCGRP I antibodyCGRP1 antibodyCGRPI antibodyCT antibodyCT antibodyKatacalcin antibodyKC antibodyMGC126648 antibody

  see all

Anti-Calcitonin antibody [16B5 ] images

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  Sandwich ELISA - Calcitonin antibody [16B5 ] (ab11493)
  Standard Curve for Calcitonin dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [16B5 ] to Calcitonin (ab11493) at 5ug/ml and Detector Antibody Rabbit polyclonal to Calcitonin (ab8553) at 0.5ug/ml
• 

  ELISA

  Calibration curves of several procalcitonin sandwich ELISAs using a range of monclonal antibodies available agaisn't procalcitonin, calcitonin and catacalcin fragments. Capture antibodies used at 1 µg/well and detection antibodies at 0.1 µg/well.

  Dark blue square = ab11493 [16B5] (calcitonin fragment) and ab11494 [42] (procalcitonin fragment). Light grey circle = ab11497 [24B2] (calcitonin fragment) and ab11484 [13B9] (calcitonin fragment). Grey triangle = ab11487 [14C12] (catacalcin fragment) and ab11496 [14A2] (calcitonin fragment). Dark grey circle = ab14813 [27A3] (procalcitonin fragment) and ab11491 [22A11] (catacalcin fragment).

• 

  Amino Acid Sequence
  Amino acid sequence and schematic diagram of human procalcitonin and the N-terminal, calcitonin, and katacalcin (catacalcin) fragments.
• 

  Western blot

  Immunodetection of human recominbinant procalcitonin in WB by a range of monoclonal antibodies available which detect different fragments of the protein, N-terminal, calcitonin or catacalcin, (see immunogen section and related figure).

  Anti- N-terminal antibodies available; 1) ab14816 [6F10], 2) ab14813 [27A3], 3) ab11498 [38F11], 4) ab14817 [44D9], 5) ab11494 [42],

  Anti- calcitonin antibodies available; 6) ab11484 [13B9], 7) ab14818 [13F2], 8) ab14819 [13G11], 9) ab11496 [14A2], 10) ab11493 [16B5], 11) ab11497 [24B2],

  Anti- catacalcin antibodies available; 12) ab11487 [14C12], 13) ab11490 [18B7], 14) ab11491 [22A11].
  

• 

  Flow Cytometry-Anti-Calcitonin antibody [16B5 ](ab11493)
  Overlay histogram showing SH-SY5Y cells stained with ab11493 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11493, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  
  

Protocols

Protocol Booklet