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Our Abpromise guarantee covers the use of ab8553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [16B5] to Calcitonin (ab11493). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab11493 as Capture.|
|ELISA||1/300 - 1/3000.|
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||1/75 - 1/150. Antigen retrieval is not essential but may optimise staining.|
|WB||1/200 - 1/500. Predicted molecular weight: 15 kDa.|
Immunohistochemical analysis of human thyroid staining with ab8553.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"