• Product nameAnti-Calcitonin antibody
    See all Calcitonin primary antibodies
  • Description
    Rabbit polyclonal to Calcitonin
  • Tested applicationsSuitable for: Sandwich ELISA, ELISA, IHC-FoFr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Horse, Dog, Pig
  • Immunogen

    Synthetic human calcitonin 1-32.

  • Positive control
    • Medullary carcinoma of the thyroid or thyroid c-cell hyperplasia.
  • General notesProlonged fixation in buffered formalin can destroy the epitope



Our Abpromise guarantee covers the use of ab8553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [16B5] to Calcitonin (ab11493). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab11493 as Capture.
ELISA 1/300 - 1/3000.
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/75 - 1/150. Antigen retrieval is not essential but may optimise staining.
WB 1/200 - 1/500. Predicted molecular weight: 15 kDa.


  • RelevanceCalcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.
  • Cellular localizationCytoplasmic and Secreted
  • Database links
  • Alternative names
    • Alpha CGRP antibody
    • alpha type CGRP antibody
    • CALC 1 antibody
    • CALC A antibody
    • CALC1 antibody
    • calcitonin 1 antibody
    • calcitonin gene related peptide 1 antibody
    • calcitonin gene related peptide I antibody
    • calcitonin related polypeptide alpha antibody
    • CGRP 1 antibody
    • CGRP antibody
    • CGRP I antibody
    • CGRP1 antibody
    • CT antibody
    • katacalcin antibody
    • KC antibody
    see all

Anti-Calcitonin antibody images

  • Standard Curve for Calcitonin dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [16B5 ] to Calcitonin (ab11493) at 5ug/ml and Detector Antibody Rabbit polyclonal to Calcitonin (ab8553) at 0.5ug/ml
  • Anti-Calcitonin antibody (ab8553) at 1/25 dilution + Human thyroid lysate.

    Predicted band size : 15 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of human thyroid staining with ab8553.


References for Anti-Calcitonin antibody (ab8553)

This product has been referenced in:
  • Yerbury JJ  et al. alpha2-Macroglobulin and haptoglobin suppress amyloid formation by interacting with prefibrillar protein species. J Biol Chem 284:4246-54 (2009). WB ; Human . Read more (PubMed: 19074141) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Horse Tissue sections (Thyroid gland)
Antigen retrieval step None
Permeabilization No
Specification Thyroid gland
Blocking step Goat normal serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 24°C
Fixative Formaldehyde


Verified customer

Submitted Mar 20 2017

Thank you for your patience. The originator of ab8553 has informed me that Calcitonin is a small peptide with 32 amino acids, MW 3417 Daltons. You also previously enquired regarding ab8571, Mouse monoclonal [ B34.1] to Thyroglobulin (FITC). Thyrogl...

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Reactivity against procalcitonin has not been specifically tested, however it may work provided the epitope recognised in calcitonin is not masked in the procalcitonin molecule. The epitope of this antibody has not been mapped, however the antibody was...

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