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Overview

  • Product nameAnti-Calcitonin antibodySee all Calcitonin primary antibodies ...
  • Description
    Rabbit polyclonal to Calcitonin
  • SpecificityThis antibody stains specifically for human parafollicular cells (C-cells) of normal and hyperplastic thyroid as well as medullary thyroid carcinoma. No staining of other cells or tissues has been observed.
  • Tested applicationsSandwich ELISA, ELISA, IHC-FoFr, IHC-P, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Horse, Dog, Pig
  • Immunogen

    Synthetic human calcitonin 1-32.

  • Positive controlMedullary carcinoma of the thyroid or thyroid c-cell hyperplasia.
  • General notesProlonged fixation in buffered formalin can destroy the epitope

Properties

Applications

Our Abpromise guarantee covers the use of ab8553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA sELISA: Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [16B5 ] to Calcitonin (ab11493). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab11493 as Capture.
ELISA ELISA: 1/300 - 1/3000.
IHC-FoFr IHC-FoFr: Use at an assay dependent dilution.
IHC-P IHC-P: 1/75 - 1/150. Antigen retrieval is not essential but may optimise staining.
WB WB: 1/200 - 1/500. Predicted molecular weight: 15 kDa.

Target

  • RelevanceCalcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.
  • Cellular localizationCytoplasmic and Secreted
  • Database links
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  • Alternative names
      Alpha CGRP antibodyalpha type CGRP antibodyCALC 1 antibody
      CALC A antibodyCALC1 antibodyCALC1 antibodyCALCA antibodycalcitonin 1 antibodycalcitonin gene related peptide 1 antibodycalcitonin gene related peptide I antibodyCalcitonin related polypeptide alpha antibodyCGRP 1 antibodyCGRP antibodyCGRP I antibodyCGRP1 antibodyCGRP1 antibodyCGRPI antibodyCT antibodyCT antibodyKatacalcin antibodyKC antibodyKC antibodyMGC126648 antibody
    see all

Anti-Calcitonin antibody images

Protocols

References for Anti-Calcitonin antibody (ab8553)

This product has been referenced in:
  • Yerbury JJ  et al. alpha2-Macroglobulin and haptoglobin suppress amyloid formation by interacting with prefibrillar protein species. J Biol Chem 284:4246-54 (2009). WB ; Human . Read more (PubMed: 19074141) »

See 1 Publication for this product

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I would like to know what is the molecular weight of the calictonin cat# ab8553, for the application of the western blot. Thanks

Thank you for your patience. The originator of ab8553 has informed me that Calcitonin is a small peptide with 32 amino acids, MW 3417 Daltons. You also previously enquired regarding ab8571, Mouse monoclonal [ B34.1] to Thyroglobulin (FITC). Thyroglo...

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does this antibody also react with procalcitonin? ulrich

Reactivity against procalcitonin has not been specifically tested, however it may work provided the epitope recognised in calcitonin is not masked in the procalcitonin molecule. The epitope of this antibody has not been mapped, however the antibody was ra...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"