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Calcium Assay Kit (Luminometric) (ab112114)

Q&A (4)

Overview

  • Product nameCalcium Assay Kit (Luminometric)See all Calcium kits ...
  • Tests
    10 x 96 well plate
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Product overview

    Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). This kit uses a highly calcium-sensitive and membrane-permeable coelenterazine analog as a calcium indicator for the cells that are transfected with apoaequorin gene. Aequorin is a calcium-sensitive bioluminescent protein from the jellyfish Aequorea victoria that has been used extensively as a calcium indicator in cells. The aequorin complex emits blue light when bound to calcium ions. The luminescence intensity is directly proportional to the Ca2+ concentration.

    ab112114 is much more sensitive than the fluorescence-based calcium assays. This kit provides an optimized assay method for monitoring the G-protein-coupled receptors and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. It might be useful for monitoring the intracellular calcium mobilization in a specified compartment given that recombinant apoaequorin proteins can now be targeted to specific organelles, cells and tissues. ab112114 is more sensitive than the fluorescent calcium assays.

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    ab112114 should be stored desiccated.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCalcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism.
    • Alternative names
        Ca++Ca2+

    Applications

    Our Abpromise guarantee covers the use of ab112114 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Calcium Assay Kit (Luminometric) images

    • Functional Studies - Calcium Assay Kit (Luminometric) (ab112114)
      Functional Studies - Calcium Assay Kit (Luminometric) (ab112114)
      ATP Dose Response on CHO-aeq cells. CHO cells stably transfected with apoaequorin were seeded overnight at 50,000 cells/100 µL/well in a white wall/clear bottom 96-well plate. The growth medium was removed and the cells were incubated with 100 µL of dye-loading solution using ab112114 for 3 hours at room temperature and protected from light. ATP (25 µL/well) was added to achieve the final indicated concentrations. The EC50 of ATP is about 0.8 µM.

    Protocols

    References for Calcium Assay Kit (Luminometric) (ab112114)

    ab112114 has not yet been referenced specifically in any publications.

    Publishing research using ab112114 ? Please let us know so that we can cite the reference in this datasheet

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    Our customer is going to purchase calcium assay kit and her sample is rat.
    I searched ab112114, ab112115 and would like to know if these kits are available to her sample.
    Please let me know.

    Calcium is essential for all living organisms.And because Calcium is an earth alkali metal but not a species specific protein, it should work with rat samples, too.

    Greetings. Thank you very much for your help and details. I really appreciate this.

    You are welcome. We are assuming the recovery period will be sufficient to allow demonstration of differences among treatments or sample types. You might consider comparing samples +/- the recovery period.

    I just found a paper that discusses an...

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    Is the assay valid for adherent cells that have been harvested before dispensing as a suspension into a 96-well plate for the assay? I am concerned that the manipulation of the samples will affect the calcium levels.

    Thank you for contacting us.

    The developer of the assay had two suggestions which may be useful. The aim in each case is to allow the cells to recover from the removal from the culture plates. The second may not be acceptable if you have a spec...

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    Is this kit suitable for tissues? How does it work?

    Thank you for your email. Unfortunately this kit is not suitable for tissues. The assay is aequorin based, and you will need to transfect the aequorin gene into the cells before the experiment. Aequorin binds Ca2+ and emits blue light, there...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"