Calcium Assay Kit (Luminometric) (ab112114)
- Product nameCalcium Assay Kit (Luminometric)See all Calcium kits ...
- Tests10 x 96 well plate
- Sample typeAdherent cells, Suspension cells
- Assay typeQuantitative
- Product overview
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). This kit uses a highly calcium-sensitive and membrane-permeable coelenterazine analog as a calcium indicator for the cells that are transfected with apoaequorin gene. Aequorin is a calcium-sensitive bioluminescent protein from the jellyfish Aequorea victoria that has been used extensively as a calcium indicator in cells. The aequorin complex emits blue light when bound to calcium ions. The luminescence intensity is directly proportional to the Ca2+ concentration.
ab112114 is much more sensitive than the fluorescence-based calcium assays. This kit provides an optimized assay method for monitoring the G-protein-coupled receptors and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. It might be useful for monitoring the intracellular calcium mobilization in a specified compartment given that recombinant apoaequorin proteins can now be targeted to specific organelles, cells and tissues. ab112114 is more sensitive than the fluorescent calcium assays.
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ab112114 should be stored desiccated.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 10 x 96 tests 100% ETOH Component B 1 x 500µl Assay Buffer 1 x 100ml Coelenterazine Analog Component A 1 vial
Our Abpromise guarantee covers the use of ab112114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Calcium Assay Kit (Luminometric) images
ATP Dose Response on CHO-aeq cells. CHO cells stably transfected with apoaequorin were seeded overnight at 50,000 cells/100 µL/well in a white wall/clear bottom 96-well plate. The growth medium was removed and the cells were incubated with 100 µL of dye-loading solution using ab112114 for 3 hours at room temperature and protected from light. ATP (25 µL/well) was added to achieve the final indicated concentrations. The EC50 of ATP is about 0.8 µM.
References for Calcium Assay Kit (Luminometric) (ab112114)
ab112114 has not yet been referenced specifically in any publications.