Overview

  • Product nameCalcium Detection Kit (Colorimetric)
    See all Calcium kits
  • Tests
    250 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    > 0.1 mM
  • Range
    0.1 mM - 25 mM
  • Assay time
    0h 20m
  • Species reactivity
    Reacts with: Cat, Human
    Predicted to work with: all Mammals
  • Product overview

    Abcam's Calcium Detection Kit (Colorimetric) utilizes the chromogenic complex (λ = 575 nm) formed between calcium ions and 0-cresolphthalein to provide a simple assay in the physiologically important range of calcium concentration 0.4-100 mg/dL (0.1-25 mM).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Calcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceCalcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism.
  • Alternative names
    • Ca++
    • Ca2+

Applications

Our Abpromise guarantee covers the use of ab102505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Calcium Detection Kit (Colorimetric) images

  • Example of standard curve obtained using ab102505

Protocols

References for Calcium Detection Kit (Colorimetric) (ab102505)

This product has been referenced in:
  • Goonasekera SA  et al. Enhanced Ca2+ influx from STIM1-Orai1 induces muscle pathology in mouse models of muscular dystrophy. Hum Mol Genet N/A:N/A (2014). Read more (PubMed: 24556214) »

See 1 Publication for this product

Product Wall



Ich habe nun einen Bestätigung von Labor: Der Farbstoff 0-cresolphthalein emittiert zwischen 540-600nm. Die spektralen Eigenschaften von 0-cresolphthalein hängen stark vom pH-Wert und der Bindung an Metallionen wie Calcium ab. Da f&...

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The blood vessel walls (largely Endothelial cells) samples can be homogenized in the assay buffer (contains detergent to help lyse cells) and then spun down to prepare a lysate. Several volumes of this lysate must be tested to make sure the r...

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The sample type of kit as mentioned on datasheet lists tissue extracts, this means the kit is suitable for any kind of tissue sections however you may need to optimize expermental conditions.

I hope this ...

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This kit can be used to detect the Calcium with rat serum and cell lysates/supernatant.

To know the price please contact our Indian distributor Allied Scientific.

Allied Scientific Products
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Fresh serum samples are recommended.

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I can confirm that this kit will detect free calcium ions only.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rab...

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We recommend to lyse the cells using the buffer provided in the kit and a Dounce homogenizer.

Thank you for contacting us.

For the preparation of the samples the source of the Calcium Detection kit suggests to use a dounce homogenizer.
Alternatively freeze/thaw cycles with vortexing in the middle can also lyse the cells, although t...

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I am happy to hear the kit finally worked as expected.

Thank you for your cooperation, and have a great week!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"