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Calcium Detection Kit (Colorimetric) (ab102505)

Submit an Abreview Q&A (19)

Overview

  • Product nameCalcium Detection Kit (Colorimetric)See all Calcium kits ...
  • Tests
    250 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    > 0.1 mM
  • Range
    0.1 mM - 25 mM
  • Assay time
    0h 20m
  • Product overview

    Abcam's Calcium Detection Kit (Colorimetric) utilizes the chromogenic complex (? = 575 nm) formed between calcium ions and 0-cresolphthalein to provide a simple assay in the physiologically important range of calcium concentration 0.4-100 mg/dL (0.1-25 mM).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Calcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCalcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism.
    • Alternative names
        Ca++Ca2+

    Applications

    Our Abpromise guarantee covers the use of ab102505 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Calcium Detection Kit (Colorimetric) images

    • Functional Studies - Calcium Detection Kit (Colorimetric) (ab102505)
      Functional Studies - Calcium Detection Kit (Colorimetric) (ab102505)
      Example of standard curve obtained using ab102505

    Protocols

    References for Calcium Detection Kit (Colorimetric) (ab102505)

    ab102505 has not yet been referenced specifically in any publications.

    Publishing research using ab102505 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab102505 Submit a Question for ab102505

    Displaying 1 - 10 of 19 results for Abreviews and Q&A

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    Inquiry: Is this kit (Calcium Detection Kit (Colorimetric) (ab102505)) suitable for determination of ca from human placenta?

    Thank you for contacting us.

    The sample type of kit as mentioned on datasheet lists tissue extracts, this means the kit is suitable for any kind of tissue sections however you may need to optimize expermental conditions.

    I hope this inf...

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    Product code: 102505
    Inquiry: Can this kit be used for rat serum or rat peritoneal macrophages (supernatant/ cell lysate)? What would be the approx price of the kit in Delhi,India?

    Thank you for contacting us.

    This kit can be used to detect the Calcium with rat serum and cell lysates/supernatant.

    To know the price please contact our Indian distributor Allied Scientific.

    Allied Scientific Products
    39...

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    Can serum be frozen before analysis with this kit, or does the serum need to be used fresh?

    Fresh serum samples are recommended.

    I know this is probably a silly question but I wanted to confirm that he calcium kit, ab10205, measures only ”free” unbound Calcium and not calcium that is bound to albumin in serum samples.

    Many thanks,

    Thank you for contacting us.

    I can confirm that this kit will detect free calcium ions only.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit...

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    I know this is probably a silly question but I wanted to confirm that he calcium kit, ab10205, measures only ”free” unbound Calcium and not calcium that is bound to albumin in serum samples.Many thanks,

    Thank you for contacting us.

    We are still working on your inquiry. We will send you the information soon.

    Many thanks for having patience!


    I need to detect intracellulary calcium in neutrophils. Do you know what sould I use to lysate the cells so that it doesn't interferes with the kit?


    We recommend to lyse the cells using the buffer provided in the kit and a Dounce homogenizer.

    protocol for sample preparation.
    sample : cell culture.
    aim : measure of calcium in cells.
    limitation : no access to sonicator.

    Thank you for contacting us.

    For the preparation of the samples the source of the Calcium Detection kit suggests to use a dounce homogenizer.
    Alternatively freeze/thaw cycles with vortexing in the middle can also lyse the cells, although this...

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    Hi,
    I used the serum intead of the plasma and it worked.
    thank you for your help.
    best regards

    I am happy to hear the kit finally worked as expected.

    Thank you for your cooperation, and have a great week!

    Hi,
    it was plasma, i used EDTA tube and centrifuged the blood.
    the excitation filter that we used was 595 nm instead of 575 nm but
    this was not the problem because as you can see we got a good standard
    curve (attached to the mail).

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    Thank you for your reply.

    Could you please confirm which anticoagulant was used to prepare plasma? For this particular kit Heparin as been shown to be the only one that worked, as others may interfere with colour development.

    If this i...

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    Hi,
    we bought the ab102505 calcium colorimetric detection kit to measure
    the calcium level in our mice blood and we are having problems,
    we followed the protocol by putting 10 microliter of mice plasma
    adding 40 microliter of water.

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    Thank you for contacting us.

    I am very sorry to hear this kit failed to give the expected results. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    In order to bette...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"