Anti-Calcium Pump pan PMCA ATPase antibody [5F10] (ab2825)

Immunoprecipitation - Calcium Pump pan PMCA ATPase antibody [5F10] (ab2825)

ab2825 Immunoprecipitate Calcium Pump pan PMCA ATPase in human whole cell lysate. 1,000,000 cells were lysed and incubated with primary antibody at 4µg/mg lysate and Protein A matrix for 20 hours at 4ºC. For western blotting an undiluted Abcam`s ab1162, Rabbit polyclonal to DDDDK tag was used.

This image is courtesy of an anonymous Abreview

Flow Cytometry-Calcium Pump pan PMCA ATPase antibody [5F10](ab2825)

Overlay histogram showing Jurkat cells stained with ab2825 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2825, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used with ab2825 (1/20 dilution) under the same conditions.

Flow Cytometry - Anti-Calcium Pump pan PMCA ATPase antibody [5F10] (ab2825)

ab2825 at a 1/200 dilution detecting Calcium Pump pan PMCA ATPase in human monocytes by Flow Cytometry. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L) used at a 1/500 dilution.

Image courtesy of Dr Mahesh Shivananjappa by Abreview.

Immunocytochemistry/ Immunofluorescence - Anti-Calcium Pump pan PMCA ATPase antibody [5F10] (ab2825)

ab2825 staining Calcium Pump pan PMCA ATPase in human monocytes by Immunocytochemistry/ Immunofluorescence.

Cells were fixed, permeabilized, blocked with 2% BSA for 30 minutes at 25°C and then incubated with ab2825 at a 1/250 dilution for 1 hour at 25°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L) used at a 1/1000 dilution.

Image courtesy of Dr Mahesh Shivananjappa by Abreview.