Calcium Quantification Assay kit (Fluorometric) (ab112115)

Overview

  • Product nameCalcium Quantification Assay kit (Fluorometric)
    See all Calcium kits
  • Tests
    1 x 200 test
  • Sample type
    Cell culture extracts
  • Assay typeQuantitative
  • Sensitivity
    0.03 mM
  • Range
    0.04 mM - 1.5 mM
  • Assay time
    0h 30m
  • Product overview

    ab112115 provides a simple method for detecting calcium in physiology solutions by using our proprietary red fluorescence probe. The fluorescence signal can be easily read by a fluorescence microplate reader at Ex/Em = 540/590 nm. The kit can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The assay can be completed within 30 minutes. With ab112115, we have detected as little as 0.03 mM calcium. The kit has a broad dynamic range (30 μM to 1 mM).


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This product is intended to be used in vitro with solution-based samples such as cell extracts. If you would like to detect calcium in vivo using live cells, please check our Fluo-8, Rhod-4 or Fura-2 based assays.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceCalcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism.
  • Alternative names
    • Ca++
    • Ca2+

Applications

Our Abpromise guarantee covers the use of ab112115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Calcium Quantification Assay kit (Fluorometric) images

  • Calcium dose response was measured on a 96-well black plate with ab112115. As low as 0.03 mM calcium can be detected with 5 minutes incubation time (n=3).

Protocols

References for Calcium Quantification Assay kit (Fluorometric) (ab112115)

ab112115 has not yet been referenced specifically in any publications.

Product Wall

Component B is a simple sodium borate buffer and therefore is just a dilution buffer.

The optimum pH for this dye is pH8.5. It is quite stable as long as it is protected from the light so you should be able to observe fluorescence after 24 hou...

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There is always some degree of non specific binding with the fluorophore and plastic of microwell plate. We are not aware if the interaction is chemical however we recommend our customer sot use negative controls i...

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Unfortunately we do not have any data to prove serum interferes with the dye. However, in order to overcome this problem you could add the same amount of serum as sample into the standard.

I hope this he...

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In regards to your questions, we would recommend freezing the stock solution instead of the reaction mix, however, there should not be a problem if it is stored at -20C and away from light for up to a week.
<...

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Merci de nous avoir contactés.

Malheureusement, le kit ab112115 ne devrait pas être utilisé dans un milieu acide. Un tampon acide pourrait conduire à des interférences et donc diminuer la fluorescence.
...

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Thank you for contacting us and your interest in our products.

The Calcium Quantification Assay kit (Fluorometric), ab112115, is intended for use with solution based samples. For your experiment, it may be better to have a look at the Fluo-8 M...

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Thank you for contacting us.

I have brought your question to the attention of the lab who have informed me that you can wait a few hours with no effect.

I hope this information is helpful to you. Please do not hesitate to cont...

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Thank you for getting back to me.

Yes, the signal would be expected to be enhanced in the presence of zinc.

And in reply to your following question, yes the Fluo-8 (ab112129) and Rhod-4 (ab112157) would not be suitable for your samp...

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Thank you for contacting us yesterday in regards to the Calcium Quantification Assay kit (Fluorometric), ab112115.

I now have some further information to share with you in regards to the kit. Unfortunately, I can confirm that the kit is likel...

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Calcium is essential for all living organisms.And because Calcium is an earth alkali metal but not a species specific protein, it should work with rat samples, too.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"