You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Calcium Quantification Assay kit (Fluorometric) (ab112115)

Q&A (13)

Overview

  • Product nameCalcium Quantification Assay kit (Fluorometric)See all Calcium kits ...
  • Tests
    1 x 200 test
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Assay time
    0h 30m
  • Product overview

    ab112115 provides a simple method for detecting calcium in physiology solutions by using our proprietary Rhod Red Indicator probe. The fluorescence signal can be easily read by a fluorescence microplate reader at Ex/Em = 540/590 nm. The kit can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The assay can be completed within 30 minutes. With ab112115, we have detected as little as 0.03 mM calcium. The kit has a broad dynamic range (30 µM to 10 mM).

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    ab112115 should be stored desiccated. Sample types culture extracts of adherent cells and suspension cells.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCalcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism.
    • Alternative names
        Ca++Ca2+

    Applications

    Our Abpromise guarantee covers the use of ab112115 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Calcium Quantification Assay kit (Fluorometric) images

    • Functional Studies - Calcium Quantification Assay kit (Fluorometric) (ab112115)
      Functional Studies - Calcium Quantification Assay kit (Fluorometric) (ab112115)
      Calcium dose response was measured on a 96-well black plate with ab112115. As low as 0.03 mM calcium can be detected with 5 minutes incubation time (n=3).

    Protocols

    References for Calcium Quantification Assay kit (Fluorometric) (ab112115)

    ab112115 has not yet been referenced specifically in any publications.

    Publishing research using ab112115 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab112115 Submit a Question for ab112115

    Displaying 1 - 10 of 13 results for Abreviews and Q&A

    10
    Votes: Highest First

    Regarding the ab112115 kit, I would like to know if you have any idea if the fluorophore used in the quit absorbs to plastics.

    Thank you very much in advance.

    Regards,

    Thank you for contacting us.

    There is always some degree of non specific binding with the fluorophore and plastic of microwell plate. We are not aware if the interaction is chemical however we recommend our customer sot use negative controls in o...

    Read More

    Hi,

    Regarding the ab112115 kit, I would like to know if you have any idea if fetal bovine serum might interfere in the measurement of the calcium in the sample solution, since some proteins from the serum can form complexes with calcium.
    ...

    Read More

    Thank you for contacting us.

    Unfortunately we do not have any data to prove serum interferes with the dye. However, in order to overcome this problem you could add the same amount of serum as sample into the standard.

    I hope this helps...

    Read More

    Hi, I purchased the ab112115 kit. I would like to know if it is possible to freeze the Assay Reaction Mixture (when the Red stock solution and the assay buffer are mixed) once we have prepare it, in aliquots, and for how long can this be preserved. In the...

    Read More

    Thank you for contacting us.

    In regards to your questions, we would recommend freezing the stock solution instead of the reaction mix, however, there should not be a problem if it is stored at -20C and away from light for up to a week.

    Read More

    Is there any interference when using an acidic pH?

    Merci de nous avoir contactés.

    Malheureusement, le kit ab112115 ne devrait pas être utilisé dans un milieu acide. Un tampon acide pourrait conduire à des interférences et donc diminuer la fluorescence.
    Read More

    Hi!

    I'm interested in the product: ab112115. My intention is to use it to measure a gradient of Ca+2 by fluorescent microscopy and study cell chimotaxis at the same time. I would like to know if this product can work in this conditions: physiolog...

    Read More

    Thank you for contacting us and your interest in our products.

    The Calcium Quantification Assay kit (Fluorometric), ab112115, is intended for use with solution based samples. For your experiment, it may be better to have a look at the Fluo-8 Medi...

    Read More

    Hi, I'm interested in using this Calcium fluorometer Ref.112115 in order to measure calcium gradient in medium and study cell chimotaxis. I'd like to know for how long does this product stay stable once you add it in the sample, I mean, how long can you w...

    Read More

    Thank you for contacting us.

    I have brought your question to the attention of the lab who have informed me that you can wait a few hours with no effect.

    I hope this information is helpful to you. Please do not hesitate to contact...

    Read More

    Dear xxxxx,

    thanks for your email and your support! So I take it that the presence of zinc would also contribute to the signal and ENHANCE the signal (and not reduce it, right?)?

    I had a look at the assay you’re mentioning&hell...

    Read More

    Thank you for getting back to me.

    Yes, the signal would be expected to be enhanced in the presence of zinc.

    And in reply to your following question, yes the Fluo-8 (ab112129) and Rhod-4 (ab112157) would not be suitable for your samples...

    Read More

    Phone call enquiring after whether the Calcium Quantification Assay kit (Fluorometric) (ab112115) is likely to cross react with zinc ions.

    Thank you for contacting us yesterday in regards to the Calcium Quantification Assay kit (Fluorometric), ab112115.

    I now have some further information to share with you in regards to the kit. Unfortunately, I can confirm that the kit is likely t...

    Read More

    Our customer is going to purchase calcium assay kit and her sample is rat.
    I searched ab112114, ab112115 and would like to know if these kits are available to her sample.
    Please let me know.

    Calcium is essential for all living organisms.And because Calcium is an earth alkali metal but not a species specific protein, it should work with rat samples, too.

    Hi I have a question about ab112115, calcium detection kit. Distilled water also contains calcium, yet it is used for dilutions in your assay. How does it affect the values determined in your assay? Thanks

    Thank you for contacting us.


    Distilled water should not contain calcium. If it does the distillation wasn't successful, please use deionised water as an alternative.

    Ihope this information is helpful to you. Please do not hesitate t...

    Read More
    

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"