Calcium Quantification Assay kit (Fluorometric) (ab112115)
- Product nameCalcium Quantification Assay kit (Fluorometric)See all Calcium kits ...
- Tests1 x 200 test
- Sample typeAdherent cells, Suspension cells
- Assay typeQuantitative
- Assay time0h 30m
- Product overview
ab112115 provides a simple method for detecting calcium in physiology solutions by using our proprietary Rhod Red Indicator probe. The fluorescence signal can be easily read by a fluorescence microplate reader at Ex/Em = 540/590 nm. The kit can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The assay can be completed within 30 minutes. With ab112115, we have detected as little as 0.03 mM calcium. The kit has a broad dynamic range (30 µM to 10 mM).
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ab112115 should be stored desiccated. Sample types culture extracts of adherent cells and suspension cells.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests 1 kit 300 mM Calcium Standard 1 x 0.5ml 1 x 0.5ml Assay Buffer 1 x 10ml 1 x 10ml Rhod Red Indicator 2 vials 2 vials
Our Abpromise guarantee covers the use of ab112115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Calcium Quantification Assay kit (Fluorometric) images
Calcium dose response was measured on a 96-well black plate with ab112115. As low as 0.03 mM calcium can be detected with 5 minutes incubation time (n=3).
References for Calcium Quantification Assay kit (Fluorometric) (ab112115)
ab112115 has not yet been referenced specifically in any publications.