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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-Calmodulin 3 antibody
See all Calmodulin 3 products (2) ...
Description
Mouse polyclonal to Calmodulin 3
Specificity
This antibody is reactive against mammalian transfected lysate.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Calmodulin 3 (AAH05137, 1 a.a. ~ 149 a.a) full-length human protein.
Positive control
Calmodulin 3 transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Signal transduction >> G protein signaling >> GPCR
Cancer >> Cell cycle >> Cell division
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Centrosome
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Cell Biology >> Cell Cycle >> Cell Division >> Cytokinesis
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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Western blot - Calmodulin 3 antibody (ab68005)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 18 , 19 kDa (predicted molecular weight: 16 kDa).
Target
Relevance
Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
Cellular localization
Spindle. Note: Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
Alternative names
- CALM antibody
- CALM2 antibody
- CALML2 antibody
see all
Database links
- Entrez Gene: 808 Human
- Omim: 114183 Human
- SwissProt: P62158 Human
- Unigene: 515487 Human
Anti-Calmodulin 3 antibody images:
Western blot - Calmodulin 3 antibody (ab68005)
All lanes : Anti-Calmodulin 3 antibody (ab68005) at 1/500 dilution
Lane 1 : Calmodulin 3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 16 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Additional bands at : 19 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Calmodulin 3 antibody (ab68005)
ab68005 has not yet been referenced specifically in any publications.
Publishing research using ab68005? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"