![Immunofluorescence - Calmodulin antibody [2D1] (ab2860) image](#ab2860_2.jpg)
![Western blot - Calmodulin antibody [2D1] (ab2860) image](#ab2860_1.jpg)
 image](#Calmodulin-Primary-antibodies-ab2860-6.jpg)
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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Overview
Product name
Anti-Calmodulin antibody [2D1]
See all Calmodulin products (9) ...
Description
Mouse monoclonal [2D1] to Calmodulin
Specificity
This antibody detects calmodulin. It does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK).By Western blot, this antibody detects a 17 kDa protein representing calmodulin from Dictyostelium cell lysate. Immunohistochemical staining of calmodulin in Dictyostelium cells with this antibody results in staining of the contractile vacuoles.
Tested applications
Flow Cyt, ELISA, WBmore details
Cross reactivity
Reacts with
Rat, Chicken, Cow, Human, Escherichia coli , Chlamydomonas reinhardtii, Dictyostelium discoideum
Immunogen
Calmodulin purified from Dictyostelium discoideum.
Positive control
WB: Purified Dictyostelium calmodulin IHC: rat brain
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Ascites
Primary antibody notes
Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.
Clonality
Monoclonal
Clone number
2D1
Isotype
IgG1
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 2µg for 106 cells.
ELISA: Use at an assay dependent dilution.
WB: 1/500Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Target
Function
Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
Sequence similarities
Belongs to the calmodulin family.
Contains 4 EF-hand domains.
Post-translational
modifications
Ubiquitination results in a strongly decreased activity.
Phosphorylation results in a decreased activity.
Cellular localization
Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
Target information above from: UniProt accessionP62158
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
There are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase.
Alternative names
- FLJ99410 antibody
- PHKD antibody
- PHKD2 antibody
see all
Database links
- Entrez Gene: 520277 Cow
- Entrez Gene: 801 Human
- Entrez Gene: 805 Human
- Entrez Gene: 808 Human
- Entrez Gene: 24242 Rat
- Entrez Gene: 24244 Rat
- Entrez Gene: 50663 Rat
- Omim: 114180 Human
see all
Anti-Calmodulin antibody [2D1] images:
Immunofluorescence - Calmodulin antibody [2D1] (ab2860)
![Immunofluorescence - Calmodulin antibody [2D1] (ab2860)](/ps/datasheet/Images/2/ab2860/ab2860_2.jpg)
Immunolocalization of calmodulin in rat brain using ab2860 (1:100)
Western blot - Calmodulin antibody [2D1] (ab2860)
![Western blot - Calmodulin antibody [2D1] (ab2860)](/ps/datasheet/Images/2/ab2860/ab2860_1.jpg)
Anti-Calmodulin antibody [2D1] (ab2860) at 1/500 dilution + Purified Dictyostelium calmodulin
Predicted band size : 17 kDa
Flow Cytometry-Anti-Calmodulin antibody [2D1](ab2860)
](/ps/datasheet/images/2/ab2860/Calmodulin-Primary-antibodies-ab2860-6.jpg)
Overlay histogram showing HeLa cells stained with ab2860 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2860, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-Calmodulin antibody [2D1] (ab2860)
This product has been referenced in:
- Boraldi Fet al. Hypoxia influences the cellular cross-talk of human dermal fibroblasts. A proteomic approach. Biochim Biophys Acta 1774:1402-13 (2007). WB; Human.Read more (PubMed: 17904921) »
- Hulen Det al. Production and specificity of monoclonal antibodies against calmodulin from Dictyostelium discoideum. Cell Motil Cytoskeleton 18:113-22 (1991).Read more (PubMed: 2013107) »
See all 2 publications for this product
Publishing research using ab2860? Please let us know so that we can cite the reference in this datasheet
![Calmodulin antibody [2D1] for Western blot in Schizosaccharomyces pombe (2860)](/ps/datasheet/images/2/ab2860/Calmodulin-Primary-antibodies-ab2860-5.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"