Anti-Calmodulin 1/2/3 antibody (ab155550)
Key features and details
- Rabbit polyclonal to Calmodulin 1/2/3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Calmodulin 1/2/3 antibody
See all Calmodulin 1/2/3 primary antibodies -
Description
Rabbit polyclonal to Calmodulin 1/2/3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Xenopus laevis, Xenopus tropicalis -
Immunogen
Recombinant fragment corresponding to Human Calmodulin 1/2/3 aa 1-165.
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Positive control
- Mouse brain whole cell lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 17 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 17 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Relevance
Function: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. -
Cellular localization
Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. -
Database links
- Entrez Gene: 808 Human
- Entrez Gene: 12315 Mouse
- Entrez Gene: 24244 Rat
- Entrez Gene: 493522 Xenopus tropicalis
- Omim: 114182 Human
- Omim: 114183 Human
- SwissProt: 114180 Human
- SwissProt: P0DP23 Human
see all -
Form
There are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase. -
Alternative names
- CALM 1 antibody
- CALM 2 antibody
- CALM 3 antibody
see all
Images
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Anti-Calmodulin 1/2/3 antibody (ab155550) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 17 kDa
15% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells labeling Calmodulin with ab155550 at 1/500 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab155550 has been referenced in 1 publication.
- Moreno C et al. Cav1.2 Activity and Downstream Signaling Pathways in the Hippocampus of An Animal Model of Depression. Cells 9:N/A (2020). PubMed: 33291797