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- Secondary antibodies
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Product name
Anti-Calmodulin antibody
See all Calmodulin products (9) ...
Description
Rabbit polyclonal to Calmodulin
Specificity
Targets C terminusof Calmodulin.
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (10-30 aa in length) conjugated to KLH and derived from the last C terminal 50 amino acids of Calmodulin (Human)
Positive control
Ramos cell lysate; Human breast carcinoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody was eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Heart disease
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Atherosclerosis >> Apoptosis
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
WB: 1/100 - 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P: Use at an assay dependent dilution.
Target
Function
Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
Sequence similarities
Belongs to the calmodulin family.
Contains 4 EF-hand domains.
Post-translational
modifications
Ubiquitination results in a strongly decreased activity.
Phosphorylation results in a decreased activity.
Cellular localization
Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
Target information above from: UniProt accessionP62158
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
There are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase.
Alternative names
- FLJ99410 antibody
- PHKD antibody
- PHKD2 antibody
see all
Database links
- Entrez Gene: 801 Human
- Entrez Gene: 805 Human
- Entrez Gene: 808 Human
- Omim: 114180 Human
- SwissProt: P62158 Human
- Unigene: 282410 Human
- Unigene: 468442 Human
- Unigene: 515487 Human
see all
Anti-Calmodulin antibody images:
Western blot - Calmodulin antibody (ab38590)
Anti-Calmodulin antibody (ab38590) + Ramos cell lysate
Predicted band size : 17 kDa
Observed band size : 17 kDa
Additional bands at : 25 kDa,30 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calmodulin antibody (ab38590)
Formalin-fixed paraffin-embedded human cancer tissue (breast carcinoma) with ab38590 anti-calmodulin, peroxidase-conjugated to the secondary antibody, followed by AEC staining.
References for Anti-Calmodulin antibody (ab38590)
This product has been referenced in:
- Cohen AA et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB; Human.Read more (PubMed: 19023046) »
See 1 publication for this product
Publishing research using ab38590? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"