Anti-Calnexin - ER membrane marker antibody [AF18] (ab31290)

Hi tech, I have a customer would like to enquire the above antibody. Can this antibody be used as loading control in my protein sample (extracted from microsomal fraction of thyroid tissue) Best regards,

ANSWER:

Thank you for contacting us and your interest in our products. I have found a reference to Calnexin being used as a loading control of microsomal fractions (link below) and although i have not found reference to it being used in thyroid tissue specifically I think it should be suitable as a loading control. http://www.jbc.org/content/285/29/22091.full I would suggest your customer search through the literature available for the protein target they are looking at and see if any alternative loading controls have been used in these studies. This can often give you an idea of what is commonly used and what may work best for your experiments. I hope this information has been of help, if you require any further information please do not hesitate ot contact us again.

Please check the below and reply me. Our customer is waiting for your shipping confirmation.    

ANSWER:

Thanks for your email. I trust you received my email yesterday which noted I have requested a free vial of ab75801 be shipped to you for your customer. I have requested a confirmation be sent as soon as possible. Please let me know if you need anything further.

Our subdealer's customer submitted complain by himself and received below email from you. Please arrange a replacement ab75801.  

ANSWER:

Sorry for the late reply. Our office was closed on Friday celebrate the Thanksgiving Day holiday.

I have initiated a request for one vial of ab75801 to be sent as a replacement. Please let me know if you have any further questions.

for your reply.    I have received the antibody within the last 6 months.  I decided to try another calnexin antibody (Cat# Ab75801) as a replacement.  So, how can I get this replacement?  I will wait for your answer.    By the way, I found an error on the protocol that I sent to you.  I used Goat anti Rabbit conjugated with Alexa Fluor 488 (Invitrogen) (4ug/ml) not Goat anti Mouse antibody, of course.  

ANSWER:

Thanks for your reply. I would be happy to set a replacement of ab75801 for you. I'm sorry but I forgot to mention that I will need to locate your order for ab31290 so I can process your request. When you have a chance, please forward me the purchase order number or Abcam order reference number so I can set up the replacement.  

Thank you for your reply.  Here I send you an image of ER staining which one actually used your another Calnexin antibody (Ab75801).  I expected connected reticular form of ER rather than puntated form of ER as i have got.  

ANSWER:

Thanks for your reply and for sending the image. I agree that there are differencees between the 2 images of ER.  Interestingly, there is an ICC/IF Abreview for ab31290 which shows staining of H1299 cells which looks more similar to the staining you are seeing. ER staining may change due to variables such as position in cell cycle, health of the cells or possibly protocol-related issues, such as using too much antibody in the ICC/IF staining. However, if you feel the vial of ab31290 is not working as expected, and have received the antibody within the last 6 months or so I would be happy to send you a replacement.