Anti-Calnexin - ER membrane marker antibody [AF18] (ab31290)

  Immunocytochemistry/ Immunofluorescence - Calnexin - ER membrane marker antibody [AF18] (ab31290)

ICC/IF image of ab31290 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab31290, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used to quench autofluorescence.  5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue).  Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calnexin - ER membrane marker antibody [AF18] (ab31290)

IHC image of Calnexin staining in human liver carcinoma FFPE section, performed on a Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31290, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.