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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
Overview
Product name
Anti-Calnexin - ER membrane marker antibody - ER Marker
See all Calnexin - ER membrane marker products (12) ...
Description
Rabbit polyclonal to Calnexin - ER membrane marker - ER Marker
Tested applications
WB, ICC, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Dog, Human, Pig, Chinese Hamster
Immunogen
His-Tag E. coli expressed N-terminal ER luminal domain of canine calnexin
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Sensory System >> Visual system
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/5000.Predicted molecular weight: 67 kDa.(1/2000 - 1/5000. Predicted molecular weight: 67 kDa; however it does run on SDS-PAGE as an ~90kDa protein, this is mainly due to a number of negative charges at the C-terminus of the protein which effects the SDS binding to the molecules and consequently its SDS-PAGE mobility.)
ICC
ICC: 1/100ICC: 1/100
ICC/IF: Use at an assay dependent concentration.
Target
Function
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.
Sequence similarities
Belongs to the calreticulin family.
Cellular localization
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP27824
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
Database links
- Entrez Gene: 403908 Dog
- Entrez Gene: 821 Human
- Entrez Gene: 12330 Mouse
- Entrez Gene: 29144 Rat
- Omim: 114217 Human
- SwissProt: P24643 Dog
- SwissProt: P27824 Human
- SwissProt: P35564 Mouse
see all
Anti-Calnexin - ER membrane marker antibody - ER Marker images:
Western blot - Calnexin - ER membrane marker antibody - ER Marker (ab10286)
Predicted band size : 67 kDa
Western blot probed with ab10286 at 1/1000.
Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines.
Lane 2 - human CEM T-lymphoblastoid leukemia cell lines.
Western blot - Calnexin antibody - ER Marker (ab10286)
Anti-Calnexin - ER membrane marker antibody - ER Marker (ab10286) at 1/1000 dilution + MCF7 cells
Secondary
Goat anti rabbit at 1/10000 dilution
Predicted band size : 67 kDa
Western blot - Calnexin - ER membrane marker antibody - ER Marker (ab10286)
All lanes : Anti-Calnexin - ER membrane marker antibody - ER Marker (ab10286) at 1/4000 dilution
Lane 1 : HeLa (Total cell lysate)
Lane 2 : HeLa (Membrane fraction)
Lane 3 : HeLa (Cytoplasmic fraction)
Lane 4 : MDA-MB231 (Total cell lysate)
Lane 5 : MDA-MB231 (Membrane fraction)
Lane 6 : MDA-MB231 (Cytoplasmic fraction)
Secondary
HRP conjugated donkey anti-rabbit
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 67 kDa
Observed band size : 67 kDa
Exposure time : 5 minutes
This image is courtesy of an Abreview submitted by Dr Neil Taylor
Immunocytochemistry/ Immunofluorescence - Calnexin - ER membrane marker antibody - ER Marker (ab10286)
Paraformaldehyde fixed HeLa cells stained with ab10286. For further details please refer to the customer Abreview.
Image taken from an Abreview submitted by Dr Neil Taylor
Immunocytochemistry/ Immunofluorescence - Calnexin - ER membrane marker antibody - ER Marker (ab10286)
ab10286 staining Calnexin in Pig retinal pigment epithelial cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/500 in 0.1% TX100; 1% goat serum; 1XPBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit IgG, dilution 1/500, was used as secondary antibody.
This image is courtesy of an Abreview submitted by Dr. Vladimir Milenkovic
References for Anti-Calnexin - ER membrane marker antibody - ER Marker (ab10286)
This product has been referenced in:
- Le Saux Oet al. Expression and In Vivo Rescue of Human ABCC6 Disease-Causing Mutants in Mouse Liver. PLoS One 6:e24738 (2011). IHC-Fr; Mouse.Read more (PubMed: 21935449) »
- Sturm Bet al. Carbamylated erythropoietin increases frataxin independent from the erythropoietin receptor. Eur J Clin Invest : (2010). WB; Human.Read more (PubMed: 20456483) »
See all 8 publications for this product
Publishing research using ab10286? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"