Calnexin - ER membrane marker antibody - ER Marker (ab13504)

Product Name 

Calnexin - ER membrane marker antibody - ER Marker

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calnexin - ER membrane marker - ER Marker

Immunogen 

Synthetic peptide: (C)EEDEILNRSPRNRKPRRE conjugated to KLH, corresponding to C terminal amino acids 575-592 of Dog Calnexin. This sequence is identical to mouse, human and rat calnexin over these residues.

BLAST '(C)EEDEILNRSPRNRKPRRE' with or

Reacts with 
(species key)

Hu, Ms, Rat, Cow, Dog, Gpig, Hm, Mk, Pig, Ql, Rb, Shp

Specificity 

Weak reactivity with Chicken, Fruitfly (Drosophila melanogaster) and Xenopus laevis.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/100.
ICC: 1/200.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution (PMID 18621691).
IP: 1/100.
WB: 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Heat Shocked HeLa Cell Lysate.

Cellular localization 

Endoplasmic reticulum

Research areas 

Neuroscience >> Sensory System >> Visual system
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER

Relevance 

Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids). These features distinguish calnexin from soluble ER chaperones that cannot interact with the transmembrane and cytosolic domains of integral membrane proteins. The amino acid sequence of calnexin is highly conserved among species, and shares regions of high sequence homology with calreticulin.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Calnexin - ER membrane marker antibody - ER Marker (ab13504)

  This product has been used in: (two most recent references)

Wang X et al. Mitochondrial degeneration and not apoptosis is the primary cause of embryonic lethality in ceramide transfer protein mutant mice. J Cell Biol 184:143-58 (2009). WB; Mouse. PubMed: 19139267

Willaert A et al. Recessive osteogenesis imperfecta caused by LEPRE1 mutations: clinical documentation and identification of the splice form responsible for prolyl 3-hydroxylation. J Med Genet 46:233-41 (2009). PubMed: 19088120

See all 12 publication references for this product.


If you publish research using ab13504 please let us know so that we can cite the reference on this datasheet.

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