Calpain 1 293T Transfected Lysate - (positive control) (ab94240)

Overview

  • Product nameCalpain 1 293T Transfected Lysate - (positive control)
  • General notesab94240 is a 293T cell transfected lysate in which Human Calpain 1 has been transiently over-expressed using a pCMV-Calpain 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWBmore details

Properties

  • BackgroundFunction: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Tissue specificity: Ubiquitous. Similarity: Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 4 EF-hand domains.

Applications

Our Abpromise guarantee covers the use of ab94240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Calpain 1 293T Transfected Lysate - (positive control) images

    • ab94240 at 15µg/lane on an SDS-PAGE gel.
    • All lanes : Anti-Calpain 1 antibody (ab89778) at 1/500 dilution

      Lane 1 : Calpain 1 293T Transfected Lysate - (positive control) (ab94240)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Calpain 1 293T Transfected Lysate - (positive control) (ab94240)

    ab94240 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Questions for ab94240.
    Please use the link above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"