Overview

  • Product nameAnti-Calpain 1 antibodySee all Calpain 1 primary antibodies ...
  • Description
    Mouse polyclonal to Calpain 1
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human Calpain 1 (NP_005177.2)

  • Positive control
    • Calpain 1 transfected 293T cell lysate, Human liver tissue lysate, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab89778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 82 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionCalcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 4 EF-hand domains.
  • Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Target information above from: UniProt accession P07384 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Ca2 activated neutral protease antibody
    • Calcium activated neutral proteinase antibody
    • Calcium activated neutral proteinase small subunit antibody
    • Calcium dependent protease small subunit 1 antibody
    • Calcium dependent protease small subunit antibody
    • Calcium-activated neutral proteinase 1 antibody
    • Calpain 1 antibody
    • Calpain 1 large subunit antibody
    • Calpain mu type antibody
    • Calpain mu-type antibody
    • Calpain regulatory subunit antibody
    • Calpain small subunit 1 antibody
    • Calpain-1 catalytic subunit antibody
    • Calpain-1 large subunit antibody
    • CAN1_HUMAN antibody
    • CANP 1 antibody
    • CANP antibody
    • CANP small subunit antibody
    • CANPL 1 antibody
    • CAPN 1 antibody
    • CAPN1 antibody
    • Cell proliferation-inducing gene 30 protein antibody
    • Micromolar Calpain antibody
    • Micromolar-calpain antibody
    • Mu Calpain antibody
    • muCANP antibody
    • muCL antibody
    see all

Anti-Calpain 1 antibody images

  • Anti-Calpain 1 antibody (ab89778) at 1 µg/ml + human liver tissue lysate at 50 µg

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
  • All lanes : Anti-Calpain 1 antibody (ab89778) at 1 µg/ml

    Lane 1 : Calpain 1 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 82 kDa
    Observed band size : 82 kDa
  • ab89778, at 10 ug/ml, staining Calpain 1 in HeLa cells by Immunofluorescence.

References for Anti-Calpain 1 antibody (ab89778)

ab89778 has not yet been referenced specifically in any publications.

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