Overview
- Product nameAnti-Calpain 1 antibodySee all Calpain 1 primary antibodies ...
- DescriptionMouse polyclonal to Calpain 1
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Human
- Immunogen
Full length Human Calpain 1 (NP_005177.2)
- Positive controlCalpain 1 transfected 293T cell lysate, Human liver tissue lysate, HeLa cells.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab89778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 82 kDa. |
| ICC/IF | ICC/IF: Use a concentration of 10 µg/ml. |
Target
- FunctionCalcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
- Tissue specificityUbiquitous.
- Sequence similaritiesBelongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains. - Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
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Database links
- Entrez Gene: 823 Human
- Omim: 114220 Human
- SwissProt: P07384 Human
- Unigene: 502842 Human
Target information above from: UniProt accession
P07384
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Ca2 activated neutral protease antibodyCalcium activated neutral proteinase antibodyCalcium activated neutral proteinase small subunit antibody
- Calcium dependent protease small subunit 1 antibodyCalcium dependent protease small subunit antibodyCalcium-activated neutral proteinase 1 antibodyCalpain 1 antibodyCalpain 1 large subunit antibodyCalpain mu type antibodyCalpain mu-type antibodyCalpain regulatory subunit antibodyCalpain small subunit 1 antibodyCalpain-1 catalytic subunit antibodyCalpain-1 large subunit antibodyCAN1_HUMAN antibodyCANP 1 antibodyCANP antibodyCANP small subunit antibodyCANPL 1 antibodyCAPN 1 antibodyCAPN1 antibodyCell proliferation-inducing gene 30 protein antibodyMicromolar Calpain antibodyMicromolar-calpain antibodyMu Calpain antibodymuCANP antibodymuCL antibody
see all
Anti-Calpain 1 antibody images
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Western blot - Calpain 1 antibody (ab89778)Anti-Calpain 1 antibody (ab89778) at 1 µg/ml + human liver tissue lysate at 50 µg
Predicted band size : 82 kDa
Observed band size : 82 kDa -
Western blot - Calpain 1 antibody (ab89778)All lanes : Anti-Calpain 1 antibody (ab89778) at 1 µg/ml
Lane 1 : Calpain 1 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 82 kDa
Observed band size : 82 kDa -
Immunocytochemistry/ Immunofluorescence - Calpain 1 antibody (ab89778)ab89778, at 10 ug/ml, staining Calpain 1 in HeLa cells by Immunofluorescence.
References for Anti-Calpain 1 antibody (ab89778)
ab89778 has not yet been referenced specifically in any publications.
