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Calpain 1 protein (Tagged-His Tag) (ab92237)

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Overview

  • Product nameCalpain 1 protein (Tagged-His Tag)See all Calpain 1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 214-500 of Human Calpain 1 with N terminal His tag; 287 amino acids, 33kDa (34kDa predicted from sequence). UniProt ID = P07384.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 121 µl distilled water.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesFEDFTGGVTEWYELRKAPSDLYQIILKALERGSLLGCSID ISSVLDMEAITFKKLVKGH AYSVTGAKQVNYRGQVVSL IRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKMED GEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTT LYEGTWRRGSTAGGCRNYPAT FWVNPQFKIRLDETDDP DDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYE VP PELVGQPAVHLKRDFFLANASRARSEQFINLREVST RFRLPPGEYVVVPST
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92237 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Ca2 activated neutral proteaseCalcium activated neutral proteinaseCalcium activated neutral proteinase small subunit
        Calcium dependent protease small subunitCalcium dependent protease small subunit 1Calcium-activated neutral proteinase 1Calpain 1Calpain 1 large subunitCalpain mu typeCalpain mu-typeCalpain regulatory subunitCalpain small subunit 1Calpain-1 catalytic subunitCalpain-1 large subunitCAN1_HUMANCANPCANP 1CANP small subunitCANPL 1CAPN 1CAPN1Cell proliferation-inducing gene 30 proteinMicromolar CalpainMicromolar-calpainMu CalpainmuCANPmuCL
      see all
  • FunctionCalcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 4 EF-hand domains.
  • Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.

    • Target information above from: UniProt accession P07384 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Calpain 1 protein (Tagged-His Tag) images

    • SDS-PAGE - Calpain 1 protein (Tagged-His Tag) (ab92237)
      SDS-PAGE - Calpain 1 protein (Tagged-His Tag) (ab92237)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Calpain 1 protein (Tagged-His Tag) (ab92237)

    ab92237 has not yet been referenced specifically in any publications.

    Publishing research using ab92237 ? Please let us know so that we can cite the reference in this datasheet

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