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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 10 antibody - Catalytic domain (Domain II)
See all Calpain 10 products (6) ...
Description
Rabbit polyclonal to Calpain 10 - Catalytic domain (Domain II)
Specificity
This antibody does not cross react with the other calpain family members (calpain 1, calpain 2, calpain 3, etc.) It recognizes latent and amino-processed Calpain 10.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Rat) based on the Catalytic domain of the large subunit. (Peptide available as ab41360.)
Epitope
This antibody binds to the middle of the catalytic domain, aminoterminal from the catalytic histidine.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Calpain 10 antibody - Catalytic domain (Domain II)(ab28220)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab28220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Detects a band of approximately 78 66 kDa (predicted molecular weight: 75 kDa).(A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection.)
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Relevance
Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. Calpain 10 is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. Calpain 10 is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Calpain-10 may represent the third example of a protease contributing to the development of diabetes, the others being prohormone-processing carboxypeptidase E and prohormone convertase-1, both of which are associated with diabetes and obesity. Multiple alternative transcript variants have been described for this gene.
Cellular localization
Cytoplasmic and Plasma membrane
Alternative names
- Calcium activated neutral proteinase 10 antibody
- CANP 10 antibody
- Calcium activated neutral protease antibody
see all
Database links
- Entrez Gene: 11132 Human
- Entrez Gene: 23830 Mouse
- Entrez Gene: 63834 Rat
- Omim: 605286 Human
- SwissProt: Q9HC96 Human
- SwissProt: Q9ESK3 Mouse
- SwissProt: Q9ES66 Rat
- Unigene: 112218 Human
see all
Anti-Calpain 10 antibody - Catalytic domain (Domain II) images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Calpain 10 antibody - Catalytic domain (Domain II)(ab28220)
IHC image of ab28220 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28220, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References for Anti-Calpain 10 antibody - Catalytic domain (Domain II) (ab28220)
ab28220 has not yet been referenced specifically in any publications.
Publishing research using ab28220? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"