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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Anti-Calpain 10 antibody - Domain III, large subunit (ab28226)
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab28226? Please let us know so that we can cite the reference in this datasheet
ab28226 has been referenced in 3 publications.
- Covington MD et al. Calpain 10 is required for cell viability and is decreased in the aging kidney. Am J Physiol Renal Physiol 296:F478-86 (2009). PubMed: 19144693
- De Maria A et al. Calpain expression and activity during lens fiber cell differentiation. J Biol Chem 284:13542-50 (2009). WB; Mouse. PubMed: 19269960
- Giguere CJ et al. Mitochondrial calpain 10 activity and expression in the kidney of multiple species. Biochem Biophys Res Commun 366:258-62 (2008). WB; Mouse, Rat, Rabbit. PubMed: 18054326
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence-Calpain 10 antibody - Domain III, large subunit(ab28226)
ICC/IF image of ab28226 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28226, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Calpain 10 antibody - Domain III, large subunit(ab28226)
IHC image of ab28226 staining in normal human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28226, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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