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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Calpain 10 peptide (Domain II and Domain III)
See all Calpain 10 products (4) ...
Protein description
This peptide was used as an immunogen for ab28226 - Calpain 10 antibody - Domain III and large subunit. By original sequence alignment of Calpain 10 with other members of the calpain family, the sequence of the ab41361 peptide falls entirely in the aminoterminal region of Domain III. More recent analysis of the Calpain 10 sequence places the first half of the ab41361 sequence in the carboxy end of the catalytic domain (Domain II) and the second half in the aminoterminal end of Domain III.
Properties
Purity
>95% by SDS-PAGE
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75
Concentration
Concentration information loading...
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
Application notes
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This peptide can be used with studies using ab28226. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Protein info
Relevance
Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. Calpain 10 is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. Calpain 10 is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Calpain-10 may represent the third example of a protease contributing to the development of diabetes, the others being prohormone-processing carboxypeptidase E and prohormone convertase-1, both of which are associated with diabetes and obesity. Multiple alternative transcript variants have been described for this gene.
Cellular localization
Cytoplasmic and Plasma membrane
References for Calpain 10 peptide (Domain II and Domain III) (ab41361)
ab41361 has not yet been referenced specifically in any publications.
Publishing research using ab41361? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"