Calpain 11 antibody - Carboxyterminal end, large subunit (ab42110)

Product Name 

Calpain 11 antibody - Carboxyterminal end, large subunit

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 11 - Carboxyterminal end, large subunit

Immunogen 

Synthetic peptide based on domain-IV in the large subunit of Human Calpain 11.

(Peptide available as ab41364.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 11 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Predicted molecular weight: 81 kDa. The latent large subunit migrates at about 81 kDa on reduced SDS PAGE gels, and truncation at the amino terminal end yields an approximately 58 kDa form. A cascade of smaller forms can be seen with further activation. ab42110 Anti Calpain-11 works well for Western Blotting applications, and when used against the reduced protein identifies bands at 81 kDa, and a series of further cleaved forms. Not all cell types produce Calpain-11; since it is a cellular protein, cell lysate works well for Western blots. Rat, mouse and human Calpain-11 are detected with ab42110. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Intracellular

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain

Relevance 

Calpain 11 is an intracellular, calcium-dependent cysteine protease. Calpain 11 was first described in the testis, and more weakly in mammary gland and thymus, and maps to chromosome 6. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV, making calpain 11 most similar to the "classical" calpains 1 & 2. Sequence alignments place calpain 11 closest to chicken calpain u/m, and it may be that calpain 11 is the human orthologue of this protein.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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