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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 11 antibody - Domain II (catalytic domain), large subunit
See all Calpain 11 products (5) ...
Description
Rabbit polyclonal to Calpain 11 - Domain II (catalytic domain), large subunit
Specificity
The antibody binds to Calpain 11, but does not cross react with the other calpain family members (calpain 1, Calpain 2, ncl 2, ncl 3, etc.) The antibody recognizes latent and the aminotruncated (active) Calpain and will also bind to the non-reduced protein.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide based on domain-III in the large subunit, using the human sequence.
(Peptide available as ab46846.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
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Western blot - Calpain 11 antibody - Domain II (catalytic domain), large subunit (ab28227)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species Predicted molecular weight: 81 kDa. The latent large subunit migrates at about 81 kD on reduced SDS PAGE gels, and truncation at the amino terminal end yields an approximately 58 Kd form. A cascade of smaller forms can be seen with further activation. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Calpain 11 is an intracellular, calcium-dependent cysteine protease. Calpain 11 was first described in the testis, and more weakly in mammary gland and thymus, and maps to chromosome 6. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV, making calpain 11 most similar to the "classical" calpains 1 & 2. Sequence alignments place calpain 11 closest to chicken calpain u/m, and it may be that calpain 11 is the human orthologue of this protein.
Cellular localization
Intracellular
Alternative names
- Calcium activated neutral proteinase 11 antibody
- CAPN 11 antibody
- CAPN11 antibody
Database links
- Entrez Gene: 11131 Human
- Entrez Gene: 268958 Mouse
- Entrez Gene: 408218 Rat
- Omim: 604822 Human
- SwissProt: Q9UMQ6 Human
- SwissProt: Q6J756 Mouse
- SwissProt: Q4V8Q1 Rat
- SwissProt: Q6IE72 Rat
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Anti-Calpain 11 antibody - Domain II (catalytic domain), large subunit images:
Western blot - Calpain 11 antibody - Domain II (catalytic domain), large subunit (ab28227)
All lanes : Anti-Calpain 11 antibody - Domain II (catalytic domain), large subunit (ab28227) at 1/1000 dilution
Lane 1 : Whole cell lysate from human testis-derived Hs1.Tes cells (treated with SF)
Lane 2 : Whole cell lysate from human testis-derived Hs1.Tes cells (treated with TNF-alpha)
Predicted band size : 81 kDa
The latent large subunit migrates at about 81 kD on reduced SDS PAGE gels, and truncation at the amino terminal end yields an approximately 58 Kd form. A cascade of smaller forms can be seen with further activation.
Protocols
References for Anti-Calpain 11 antibody - Domain II (catalytic domain), large subunit (ab28227)
ab28227 has not yet been referenced specifically in any publications.
Publishing research using ab28227? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"