Anti-Calpain 11 antibody - Domain IV (EF-1), large subunit (ab28228)

Application notes

WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. Predicted molecular weight: 81 kDa. The latent large subunit migrates at about 81 kD on reduced SDS PAGE gels, and truncation at the amino terminal end yields an approximately 58 kD form. A cascade of smaller forms can be seen with further activation. The antibody works well for Western Blotting applications, and when used against the reduced protein identifies bands at 81 kD, and a series of further cleaved forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.