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Product Name
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Calpain 11 peptide (Catalytic domain (Domain II))
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Product type
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Proteins and Peptides
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Peptide
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This peptide was used as an immunogen for ab28227 - Calpain 11 antibody - Domain II (catalytic domain) and large subunit.
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Specificity
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We have a range of domain specific antibodies for this target. For a full list please see all Calpain 11 antibodies
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Tested applications
(see key)
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ELISA, WB
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This peptide can be used with studies using ab28227. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
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Relevance
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Calpain 11 is an intracellular, calcium-dependent cysteine protease. Calpain 11 was first described in the testis, and more weakly in mammary gland and thymus, and maps to chromosome 6. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV, making calpain 11 most similar to the "classical" calpains 1 & 2. Sequence alignments place calpain 11 closest to chicken calpain u/m, and it may be that calpain 11 is the human orthologue of this protein.
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: None
Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Calpain 11 peptide (Catalytic domain (Domain II)) is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Calpain 11
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Calpain 11 peptide (Catalytic domain (Domain II)) - more information
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