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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Overview
Product name
Anti-Calpain 14 antibody - Domain I, large subunit
See all Calpain 14 products (2) ...
Description
Rabbit polyclonal to Calpain 14 - Domain I, large subunit
Specificity
ab38945 recognizes latent but not the aminotruncated (active) Calpain 14. It binds to Calpain 14, but does not cross react with the other calpain family members (Calpain 1, Calpain 2, ncl 2, ncl 3, etc.)
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide based on the aminoterminal end of domain-I in the large subunit of Human Calpain 14. (Peptide available as ab41371.)
General notes
Store diluted antibody at 4°C with 0.05% Sodium Azide as preservative.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab38945 has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. When used against the reduced protein this antibody identifies bands at 76 kD, and a series of further cleaved forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Calcium-regulated non-lysosomal thiol-protease.
Tissue specificity
Not expressed in tissues tested.
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 3 EF-hand domains.
Target information above from: UniProt accessionA8MX76
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Calcium-activated neutral proteinase 14 antibody
- Calpain-14 antibody
- CAN14_HUMAN antibody
see all
Database links
- Entrez Gene: 440854 Human
- Omim: 610229 Human
- SwissProt: A8MX76 Human
- Unigene: 468059 Human
Protocols
References for Anti-Calpain 14 antibody - Domain I, large subunit (ab38945)
ab38945 has not yet been referenced specifically in any publications.
Publishing research using ab38945? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"