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Product Name
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Calpain 14 peptide (Domain I)
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Product type
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Proteins and Peptides
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Peptide
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This peptide was used as an immunogen for ab38945 - Calpain 14 antibody - Domain I and large subunit.
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Specificity
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We have a range of domain specific antibodies for this target. For a full list please see all Calpain 14 antibodies
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Tested applications
(see key)
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ELISA, WB
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This peptide can be used with studies using ab38945. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
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Relevance
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Calpains are used as markers of traumatic brain injury (Pineda, 2004). Calpain 14 is an intracellular, calcium-dependent cysteine protease. Calpain 14 was first described in human EST databases, and maps to mouse chromosome 2. The 663 amino acid sequence predicts a protein of 76.749 kD, with a isoelectric point of 8.587 (basic compared to the acidic isoelectric point of “classical” calpains). Calpain 14 looks similar to the other calpain members in domains I and II, but domain III lacks the polyglutamic acid stretch that aids in calcium binding, and domain IV is very different. Sequence alignments place Calpain-14 closest to calpain 13, also found on chromosome 2.
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: None
Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Calpain 14 peptide (Domain I) is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Calpain 14
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Calpain 14 peptide (Domain I) - more information
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Customer reviews (feedback) regarding Calpain 14 peptide (Domain I) |
Customer FAQs regarding Calpain 14 peptide (Domain I) |
Protocols for Calpain 14 peptide (Domain I) |
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