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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 2 antibody [107-82]
See all Calpain 2 products (10) ...
Description
Mouse monoclonal [107-82] to Calpain 2
Specificity
Detects the 80 kDa subunit of Calpain 2 in human platelets and erythrocytes; bovine platelets, heart and skeletal muscle; rat myoblasts, kidney, liver and spleen; and cultured pig cells. This antibody does not cross-react with Mu Calpain, N Calpain, calmodulin or calpastatin.
Tested applications
Flow Cyt, ICC, WBmore details
Cross reactivity
Reacts with
Rat, Cow, Human, Pig
Immunogen
Purified bovine skeletal muscle 80 kDa subunit of Calpain 2.
Epitope
This antibody recognizes an epitope between amino acids 502-699 (domain III / IV) of human Calpain 2.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
107-82
Isotype
IgG1
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 80 kDa.
Target
Function
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 3 EF-hand domains.
Cellular localization
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
Target information above from: UniProt accessionP17655
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Calcium activated neutral proteinase 2 antibody
- Calcium activated neutral proteinase antibody
- Calcium-activated neutral proteinase 2 antibody
see all
Database links
- Entrez Gene: 281662 Cow
- Entrez Gene: 824 Human
- Entrez Gene: 29154 Rat
- Omim: 114230 Human
- SwissProt: Q27971 Cow
- SwissProt: P17655 Human
- SwissProt: P43367 Pig
- SwissProt: Q07009 Rat
see all
References for Anti-Calpain 2 antibody [107-82] (ab3588)
ab3588 has not yet been referenced specifically in any publications.
Publishing research using ab3588? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"