![Western blot - Calpain 2 antibody [1E8] (ab713) image](#ab713_1.gif)
![Immunocytochemistry/ Immunofluorescence - Calpain 2 antibody [1E8] (ab713) image](#ab713_2.gif)
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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 2 antibody [1E8]
See all Calpain 2 products (10) ...
Description
Mouse monoclonal [1E8] to Calpain 2
Specificity
Recognises a linear epitope of the 80 kDa (large) subunit of bovine m-calpain
Tested applications
ICC/IF, ELISA, WBmore details
Cross reactivity
Reacts with
Cow, Human
Immunogen
Bovine cardiac Calpain 2.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
1E8
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Applications
Show applications key
Our Abpromise guarantee covers the use of ab713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 0.5 µg/ml(Fix with 2% paraformaldehyde.)
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/ml
Target
Function
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 3 EF-hand domains.
Cellular localization
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
Target information above from: UniProt accessionP17655
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Calcium activated neutral proteinase 2 antibody
- Calcium activated neutral proteinase antibody
- Calcium-activated neutral proteinase 2 antibody
see all
Database links
- Entrez Gene: 281662 Cow
- Entrez Gene: 824 Human
- Omim: 114230 Human
- SwissProt: Q27971 Cow
- SwissProt: P17655 Human
- Unigene: 350899 Human
Anti-Calpain 2 antibody [1E8] images:
Western blot - Calpain 2 antibody [1E8] (ab713)
![Western blot - Calpain 2 antibody [1E8] (ab713)](/ps/datasheet/Images/0/ab713/ab713_1.gif)
Immunodetection of m-calpain using MAb 1E8 (1) in Western blot.
Immunocytochemistry/ Immunofluorescence - Calpain 2 antibody [1E8] (ab713)
![Immunocytochemistry/ Immunofluorescence - Calpain 2 antibody [1E8] (ab713)](/ps/datasheet/Images/0/ab713/ab713_2.gif)
Immunoflurescence of intracellular calpain in bovine lens epithel cells using MAb 1E8 (green).
References for Anti-Calpain 2 antibody [1E8] (ab713)
ab713 has not yet been referenced specifically in any publications.
Publishing research using ab713? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"