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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 2 antibody - Aminoterminal end domain I
See all Calpain 2 products (10) ...
Description
Rabbit polyclonal to Calpain 2 - Aminoterminal end domain I
Specificity
This antibody reacts with the propeptide domain, (Domain I) of Calpain 2, aminoterminal from the activation cleavage site. It binds to reduced and non reduced Calpain 2, but does not recognize amino processed Calpain 2. The antibody does not cross react with the other calpain family members (m calpain, calpain 94, ncl 2, ncl 3, etc.).
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide based on the amino terminal end of the large subunit (Domain I) of Human Calpain 2.
Positive control
Cell lysates from human fetal fibroblasts.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
This antibody could be used to discriminate between latent and amino processed forms of Calpain 2, as it does not recognize amino processed Calpain 2.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
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Immunocytochemistry/ Immunofluorescence-Calpain 2 antibody - Aminoterminal end domain I(ab39167)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab39167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).(Dilution optimised using Chromogenic detection.)
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 3 EF-hand domains.
Cellular localization
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
Target information above from: UniProt accessionP17655
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Calcium activated neutral proteinase 2 antibody
- Calcium activated neutral proteinase antibody
- Calcium-activated neutral proteinase 2 antibody
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Database links
- Entrez Gene: 824 Human
- Entrez Gene: 12334 Mouse
- Entrez Gene: 29154 Rat
- Omim: 114230 Human
- SwissProt: P17655 Human
- SwissProt: O08529 Mouse
- SwissProt: Q07009 Rat
- Unigene: 350899 Human
see all
Anti-Calpain 2 antibody - Aminoterminal end domain I images:
Immunocytochemistry/ Immunofluorescence-Calpain 2 antibody - Aminoterminal end domain I(ab39167)
ICC/IF image of ab39167 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39167, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-Calpain 2 antibody - Aminoterminal end domain I (ab39167)
This product has been referenced in:
- Ozaki Tet al. Mitochondrial m-calpain plays a role in the release of truncated apoptosis-inducing factor from the mitochondria. Biochim Biophys Acta 1793:1848-59 (2009). WB; Rat.Read more (PubMed: 19833151) »
See 1 publication for this product
Publishing research using ab39167? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"