Overview
- Product nameAnti-Calpain 2 antibodySee all Calpain 2 primary antibodies ...
- DescriptionRabbit polyclonal to Calpain 2
- Specificityab28259 recognises the Carboxyterminal end of the large subunit of calpain 2, both in its latent and aminoprocessed (activated) forms. It does not recognise the other calpain family members (calpain 1, calpain 94, ncl 2, ncl 3, etc.).
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide based on the Carboxyterminal end of the large subunit of human calpain 2. (Peptide available as ab41303.)
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 50% Glycerol -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab28259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000 - 1/5000. Predicted molecular weight: 80 kDa.Can be blocked with Calpain 2 peptide (Domain IV) (ab41303). 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. But note that when used against the reduced protein, ab28259 identifies bands at 80 kDa, 58 kDa and a series of further cleaved active forms. Dilution optimised using Chromogenic detection. |
Target
- FunctionCalcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
- Tissue specificityUbiquitous.
- Sequence similaritiesBelongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 3 EF-hand domains. - Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
-
Database links
- Entrez Gene: 824 Human
- Entrez Gene: 12334 Mouse
- Entrez Gene: 29154 Rat
- Omim: 114230 Human
- SwissProt: P17655 Human
- SwissProt: O08529 Mouse
- SwissProt: Q07009 Rat
- Unigene: 350899 Human
- Unigene: 19306 Mouse
- Unigene: 6822 Rat
see all
Target information above from: UniProt accession
P17655
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- Calcium activated neutral proteinase 2 antibodyCalcium activated neutral proteinase antibodyCalcium-activated neutral proteinase 2 antibody
- CALP80 antibodyCalpain 2 (m/II) large subunit antibodyCalpain 2 catalytic subunit antibodyCalpain 2 large catalytic subunit antibodyCalpain 2 large subunit antibodyCalpain 2, large [catalytic] subunit antibodyCalpain large polypeptide L2 antibodyCalpain M type antibodyCalpain M-type antibodyCalpain-2 catalytic subunit antibodyCalpain-2 large subunit antibodyCalpain2 antibodyCAN2_HUMAN antibodyCANP 2 antibodyCANP L2 antibodyCANP2 antibodyCANPL 2 antibodyCANPL2 antibodyCANPml antibodyCapa2 antibodyCAPN 2 antibodyCAPN2 antibodyCAPN2 antibodyFLJ39928 antibodyM calpain antibodyM calpin antibodyM type antibodyM-calpain antibodymCANP antibodyMillimolar calpain antibodyMillimolar-calpain antibody
see all
Anti-Calpain 2 antibody images
-
Western blot - Calpain 2 antibody - Domain IV (ab28259)All lanes : Anti-Calpain 2 antibody (ab28259) at 1/1000 dilution
Lane 1 : Calpain control
Lane 2 : Cell lysates from human chodrosarcoma (treated with II1a)
Predicted band size : 80 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 36 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Protocols
References for Anti-Calpain 2 antibody (ab28259)
This product has been referenced in:
- Ozaki T et al. Mitochondrial m-calpain plays a role in the release of truncated apoptosis-inducing factor from the mitochondria. Biochim Biophys Acta 1793:1848-59 (2009). WB ; Rat . Read more (PubMed: 19833151) »
