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All lanes : Anti-Calpain 3 antibody [Calp3c/11B3] (ab51859) at 1/100 dilution
Lane 1 :
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 94 kDa
Observed band size : 84 kDa (why is the actual band size different from the predicted?)
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