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Anti-Calpain 3 antibody [Calp3c/11B3] (ab51859)

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Overview

Product name

Anti-Calpain 3 antibody [Calp3c/11B3]
See all Calpain 3 products (13) ...

Description

Mouse monoclonal [Calp3c/11B3] to Calpain 3

Specificity

ab51859 reacts with calpain 3 (94kD) plus pre-and post-autolysed forms of the ubiquitous calpains 1 and 2 (µ and m-calpains).

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Rabbit, Chicken, Human, Pig

Immunogen

Synthetic peptide: RLRNPWGQVEWNGSWS, corresponding to amino acids 355-370 of Human Calpain 3

RLRNPWGQVE WNGSWS

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Freshly isolated rat or normal human skeletal muscle homogenate prepared in treatment buffer containing 0.125M tris-HCI buffer pH6.4, 10% glycerol, 4% SDS, 4M urea, 10% mercaptoethanol and 0.001% bromophenol blue (final pH of treatment buffer, 6.8). Staining pattern Bands of approximately 94, 80 and 60kD in rat muscle extracts and/or bands of approximately 78 and 94kD in Western blots of human muscle extracts.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

Calp3c/11B3

Myeloma

x63-Ag8.653

Isotype

IgG2a

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain

  • Western blot - Calpain 3 antibody [Calp3c/11B3] (ab51859)Western blot - Calpain 3 antibody [Calp3c/11B3] (ab51859) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab51859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Calcium-regulated non-lysosomal thiol-protease.

Tissue specificity

Isoform I is skeletal muscle specific.

Involvement in disease

Defects in CAPN3 are the cause of limb-girdle muscular dystrophy type 2A (LGMD2A) [MIM:253600]. LGMD2A is an autosomal recessive degenerative myopathy characterized by progressive symmetrical atrophy and weakness of the proximal limb muscles and elevated serum creatine kinase. The symptoms usually begin during the first two decades of life, and the disease gradually worsens, often resulting in loss of walking ability 10 or 20 years after onset.

Sequence similarities

Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP20807 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Calcium activated neutral proteinase 3 antibody
  • Calcium-activated neutral proteinase 3 antibody
  • Calpain L3 antibody
  • Calpain large polypeptide L3 antibody
  • Calpain p94 antibody
  • Calpain p94 large catalytic subunit antibody
  • Calpain-3 antibody
  • Calpain3 antibody
  • CalpainL3 antibody
  • Calpainp94 antibody
  • CAN3_HUMAN antibody
  • CANP 3 antibody
  • CANP3 antibody
  • CANPL 3 antibody
  • CANPL3 antibody
  • CAPN 3 antibody
  • CAPN3 antibody
  • LGMD 2 antibody
  • LGMD 2A antibody
  • LGMD2 antibody
  • LGMD2A antibody
  • Limb girdle muscular dystrophy 2A antibody
  • Lp82 antibody
  • Lp85 antibody
  • MGC10767 antibody
  • MGC11121 antibody
  • MGC14344 antibody
  • MGC4403 antibody
  • Muscle specific calcium activated neutral protease 3 antibody
  • Muscle specific calcium activated neutral protease 3 large subunit antibody
  • Muscle-specific calcium-activated neutral protease 3 antibody
  • nCL 1 antibody
  • nCL-1 antibody
  • nCL1 antibody
  • New calpain 1 antibody
  • p94 antibody
see all

Database links

see all

Anti-Calpain 3 antibody [Calp3c/11B3] images:

  Western blot - Calpain 3 antibody [Calp3c/11B3] (ab51859)

Western blot - Calpain 3 antibody [Calp3c/11B3] (ab51859)

All lanes : Anti-Calpain 3 antibody [Calp3c/11B3] (ab51859) at 1/100 dilution

Lane 1 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size : 94 kDa
Observed band size : 84 kDa (why is the actual band size different from the predicted?)

References for Anti-Calpain 3 antibody [Calp3c/11B3] (ab51859)

ab51859 has not yet been referenced specifically in any publications.

Publishing research using ab51859? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"